Evaluation of the Effects of Iron Oxide Nanoparticles on Expression of TEM Type Beta-Lactamase Genes in Pseudomonas Aeruginosa
Mina
Lotfpour
Department of Microbiology, School of Basic Sciences, Saveh Branch, Islamic Azad University, Saveh, Iran.
author
Kumarss
Amini
Department of Microbiology, School of Basic Sciences, Saveh Branch, Islamic Azad University, Saveh, Iran.
author
text
article
2020
eng
Pseudomonas aeruginosa is a common cause of surgical-site infections and healthcare-associated infections in the bloodstream, and urinary tract. Iron oxide nanoparticles (IONPs) have shown, to possess antibacterial features. The nanoparticles' status as emerging therapeutic elements has motivated investigators to assess the effects of iron nanoparticles on the expression of TEM type beta-lactamase genes in P. aeruginosa. In this descriptive-analytic study, 60 clinical isolates of P. aeruginosa were isolated from burn wounds and respiratory excretions of Pasargad Research Laboratory of Tehran, Iran. All isolates were characterized using differential biochemical tests and confirmed samples as P. aeruginosa. Their genomic DNA was extracted and PCR reaction was performed to screen TEM-gene carrying isolates. Then MIC of IONPs against these strains was determined and finally, Real-time PCR performed to the determination of the expression of the TEM gene. Results showed that 8 isolates (13/33%) had the TEM beta-lactamase gene. The MIC and MBC of IONPs against P. aeruginosa strains were observed at 256 µg/mL or 125 µg/mL, while the MBC was determined at 500 µg/mL. In addition, statistical analysis of Real-time PCR data showed that there is a statistically significant difference between gene expression levels of IONPs treated isolates and non-treated ones. The results showed that TEM gene expression levels in two isolates treated with IONPs were 78% and 75% lower than untreated bacteria (P<0.001; r= 0.958). Our findings confirmed that IONPs are potential antibacterial agents and can be considered as promising treatments for recalcitrant P. aeruginosa infections.
Journal of Sciences, Islamic Republic of Iran
University of Tehran
1016-1104
31
v.
2
no.
2020
105
111
https://jsciences.ut.ac.ir/article_76400_0e50fcee5a8235131e1d5d6d0d5a8622.pdf
dx.doi.org/10.22059/jsciences.2020.289682.1007450
Epithelial-Mesenchymal Transition and Inflammation in Head and Neck Squamous Cell Carcinoma
Habib
Sadeghi Rad
Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
author
Abbas
Shakoori Garakani
Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, Tehran. I.R Iran.
author
Ghodratolah
Panahi
Department of Biochemistry, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, I.R Iran.
author
Saman
Mehrabi
Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, Tehran. I.R Iran.
author
Mohammad reza
Noori daloii
Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
author
text
article
2020
eng
Head and neck squamous cell carcinoma (HNSCC) represents a large majority of cancers arising from the head and neck, especially the oral cavity. Despite advances in therapy, the five-year survival rate remains low due to the number of patients presenting advanced stages of the disease. The role of epithelial-mesenchymal transition (EMT) in tumorigenesis in HNSCC remains unexplored. The current study aimed at investigating the mRNA expression of the three major factors in tumor specimens to define their functional and pathological roles in this malignancy. The expression of E-cadherin, vimentin, and tumor necrosis factor (TNF)-α were examined in 31 tumorous and 31 non-tumorous samples obtained from patients with HNSCC. Total RNA was extracted from all tumors for cDNA synthesis and mRNA expression levels of E-cadherin, vimentin, and TNF-α were assessed by real-time PCR. We showed a significant decrease in E-cadherin expression and increase in vimentin and TNF-α expression in tumorous samples in comparison with non-tumorous ones (P ≤0.05). Also, there was a significant correlation between vimentin mRNA expression and poor differentiation of tumor (P ≤0.05).Since many studies investigated EMT markers in head and neck cell lines, the current study on human samples can unveil the role of these markers in HNSCC and their relationship with patients` clinicopathological features. Therefore, it might be possible to prevent it, and a therapeutic strategy could be effective in the future.
Journal of Sciences, Islamic Republic of Iran
University of Tehran
1016-1104
31
v.
2
no.
2020
113
119
https://jsciences.ut.ac.ir/article_76401_3e5a236bb826d3b7aa15bcf67ce16b34.pdf
dx.doi.org/10.22059/jsciences.2020.295886.1007482
Molecular Interaction Studies of Ammonium Dihydrogen Phosphate in the Aqueous Solutions of Mono, Di and Triprotic Acids – An Ultrasonic Study
S
Prabagaran
Department of physics, Sacred heart College, Tirupattur - 635601, Vellore District, Tamil Nadu, India
author
S
Venkatesan
Department of Chemistry, Adhiyamaan College of Engineering, Hosur, India
author
J
Udayaseelan
Department of Physics, Government Thirumagal Mills College, Gudiyattam- 632 602, Tamil Nadu, India
author
M
Aravinthraj
Department of Physics,
Sacred Heart College
Tirupattur - 635601, Vellore District, Tamil Nadu, India
author
text
article
2020
eng
Aqueous solutions of mono, di, and triprotic acids were prepared with different molar percentages (say 10%, 20% and 30%). ammonium dihydrogen phosphate is gradually introduced in the aqueous solutions at different concentrations varied from 0.1M to 0.6M at 303K. Densities, viscosities, and velocity of sound of aqueous solutions are measured and they are used to evaluate the other parameters like adiabatic compressibility (β), free length (Lf), internal pressure (πi), apparent molar volume (φV), apparent molar compressibility (φK) and relative Association (RA) of the solution. Experimental results are analyzed based on molecular associations like hydrogen bonding and dipole-dipole interactions between the component molecules, which revealed that the variation of the parameters with the different molar concentrations of both ammonium dihydrogen phosphate and the acids.
Journal of Sciences, Islamic Republic of Iran
University of Tehran
1016-1104
31
v.
2
no.
2020
121
126
https://jsciences.ut.ac.ir/article_74797_a3ab77f5b23dab8484933371c39a6eac.pdf
dx.doi.org/10.22059/jsciences.2020.280203.1007400
Investigation of Catalytic Activity of Pillared-layer Ni (II) Metal-Organic Framework Derived NiO Nanoparticles for Aromatization of Hantzsch 1,4-Dihydropyridines
Zeinab
Asgharpour
Alzahra University
author
Faezeh
Farzaneh
Al Zahra University
author
text
article
2020
eng
The pillared-layer metal-organic framework of Ni2(BTEC)(bipy)3.3DMF.2H2O (BTEC = 1,2,4,5-benzenetetracarboxylate; bipy = 4,4́-bipydine; DMF =N,Ń-dimethylformamide) was prepared, characterized and used as a precursor for preparation of NiO nanoparticles. The morphology and structure of NiO nanoparticles were characterized by XRD, SEM, FT-IR and EDX techniques. It was found that the synthesized MOF and NiO nanoparticles catalyze the aromatization of 4-substituted Hantzsch 1,4-dihydropyridines (R= Ph, Me, H) with 100% conversion and 100% selectivity toward the desired products.
Journal of Sciences, Islamic Republic of Iran
University of Tehran
1016-1104
31
v.
2
no.
2020
127
135
https://jsciences.ut.ac.ir/article_74787_17b819f4cc328c216fb9b4b8ec2e343d.pdf
dx.doi.org/10.22059/jsciences.2020.291546.1007461
DFT Study and NBO Analysis of Conformation Properties of 2,5,5-Trimethyl-1,3,2-Dioxaphosphinane 2-Selenide and Their Dithia and Diselena Analogous
Nasrin
Masnabadi
Department of Chemistry, Roudehen Branch, Islamic Azad University, Roudehen, Iran
author
text
article
2020
eng
The hybrid density functional theory (B3LYP) and ab initio molecular orbital (HF) based methods and Natural Bond Orbital (NBO) interpretation were used to analyze the conformational behaviors of 2,5,5-trimethyl-1,3,2-dioxaphosphinane 2-selenide (compound 1), 2,5,5-trimethyl-1,3,2-dithiaphosphinane 2-selenide (compound 2) and 2,5,5-trimethyl-1,3,2-diselena phosphinane 2-selenide (compound 3). The results explained that the axial conformations had a preference of compounds 1-3. The relative energies ∆E0 and Gibbs free energy difference values (ΔGeq-ax) between the ax and eq-conformations were calculated and showed the decrease from compound 1 to 3. Stereo electronic effect (SE) for 1 to 3 has been calculated by the NBO analysis. Therefore, in this study, other factors such as stereo electronic effects, electrostatic and steric interactions of compounds 1 to 3 conformational behavior have been evaluated. One examined whether the stereo electronic effect is the only factor affecting the conformational behavior or not? The electronic properties such as the HOMO and LUMO energies were also determined to investigate the reactive sites of the compounds. Structural-relative activities of compounds are also evaluated.
Journal of Sciences, Islamic Republic of Iran
University of Tehran
1016-1104
31
v.
2
no.
2020
137
146
https://jsciences.ut.ac.ir/article_76402_b9932f5e8a92a356afca7c2d796e4a4b.pdf
dx.doi.org/10.22059/jsciences.2020.289819.1007453
Synthesis and in vitro Anti-Bacterial Activity of 2-(5-Nitro-2-heteroaryl)-1,3,4-Thiadiazole Derivatives
M.
Yazdanian
Kerman University of Medical Sciences
author
B
Zihayat
Kerman University of Medical Sciences
author
MH
Moshafi
Kerman University of Medical Sciences
author
A
Khodadadi
Kerman University of Medical Sciences
author
A
Peimani
Kerman University of Medical Sciences
author
A
Shamsaddini
Kerman University of Medical Sciences
author
M
Nourhashemi
Tehran University of Medical Sciences
author
Alireza
Foroumadi
Tehran University of Medical Sciences
author
text
article
2020
eng
A new series of 2-(5-nitro-2-heteroaryl)-1,3,4-thiadiazole derivatives, including nitro furan, nitro thiophene and nitro imidazole, were synthesized and screened in vitro for their inhibitory activity against eight bacterial strains. The results showed that most of the synthesized compounds were active against Gram-positive bacteria, determined by MIC method. Among them, compounds 6a, 6b and 6d exhibited strong anti-bacterial effects against the Gram-positive bacteria. The oxidation of synthesized compounds to sulfinyl and sulfonyl bearing analogues did not improve the activity.
Journal of Sciences, Islamic Republic of Iran
University of Tehran
1016-1104
31
v.
2
no.
2020
147
153
https://jsciences.ut.ac.ir/article_74789_4ef3a45675e366fcbdf240d9dd9c8cbd.pdf
dx.doi.org/10.22059/jsciences.2020.283309.1007419
Early Silurian (Llandovery) Trilobite fauna from
Hamed
Ameri
Department of Ecology, Institute of Science, High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman,
author
text
article
2020
eng
The aim of this study is to analyze the assemblage of trilobites in the Silurian sequences of Iran by exploring stratigraphic section of the Silurian Niur Formation (Asadli section). Based on comprehensive field studies and laboratory investigations three Trilobite genera were identified in the study area including Calymene blumenbachii, Gravicalymene convolva, Dalmanites brevigladiolus and Calymene sp. according to this trilobite assemblage a Rhuddanian to Telychian stages (Llandovery) is suggested for deposition of the study strata. The recognized trilobite fauna will be used as a base for future geological studies on the Silurian strata in Kopeh-Dagh Basin. This is the first report of trilobites from the Silurian (Llandovery) Niur Formation in Asadli section, north- east, Iran.
Journal of Sciences, Islamic Republic of Iran
University of Tehran
1016-1104
31
v.
2
no.
2020
155
164
https://jsciences.ut.ac.ir/article_74792_c84d5636fe5bbf99d4e2be825c9b927b.pdf
dx.doi.org/10.22059/jsciences.2020.275984.1007374
A Classification Method for E-mail Spam Using a Hybrid Approach for Feature Selection Optimization
Zeinab
Hassani
Department of computer science, Kosar University of Bojnord, Iran.
author
vahid
Hajihashemi
Student Member, IEEE
author
Keivan
Borna
Faculty of Mathematics and Computer Science, Kharazmi University, Tehran, IRAN
author
Iman
Sahraei Dehmajnoonie
Science and Research Branch, Islamic Azad University, kerman, Iran
author
text
article
2020
eng
Spam is an unwanted email that is harmful to communications around the world. Spam leads to a growing problem in a personal email, so it would be essential to detect it. Machine learning is very useful to solve this problem as it shows good results in order to learn all the requisite patterns for classification due to its adaptive existence. Nonetheless, in spam detection, there are a large number of features to attend as they play an essential role in detection efficiency. In this article, we're working on a feature selection method to e-mail spam. This approach is considered a hybrid of optimization algorithms and classifiers in machine learning. Binary Whale Optimization (BWO) and Binary Grey Wolf Optimization (BGWO) algorithms are used for feature selection and K-Nearest Neighbor (KNN) and Fuzzy K-Nearest Neighbor (FKNN) algorithms are applied as the classifiers in this research. The proposed method is tested on the "SPAMBASE" datasets from UCI Machine learning Repesotries and the experimental results revealed the highest accuracy of 97.61% on this dataset. The obtained results indicateed that the proposed method is suitable and capable to provide excellent performance in comparison with other methods.
Journal of Sciences, Islamic Republic of Iran
University of Tehran
1016-1104
31
v.
2
no.
2020
165
173
https://jsciences.ut.ac.ir/article_74790_61ab0c03859ddd061039068b5286b726.pdf
dx.doi.org/10.22059/jsciences.2020.288729.1007444
Use of Two Smoothing Parameters in Penalized Spline Estimator for Bi-variate Predictor Non-parametric Regression Model
Anna
Islamiyati
Department of Statistics Faculty of Mathematics and Natural Sciences Hasanuddin University, Makassar, Sulawesi Selatan, Indonesia.
author
text
article
2020
eng
Penalized spline criteria involve the function of goodness of fit and penalty, which in the penalty function contains smoothing parameters. It serves to control the smoothness of the curve that works simultaneously with point knots and spline degree. The regression function with two predictors in the non-parametric model will have two different non-parametric regression functions. Therefore, we propose the use of two smoothing parameters in the bi-variate predictor non-parametric regression model. We demonstrated its ability through longitudinal data simulation studies with a comparison of one smoothing parameter. It was done on several numbers of subjects with repeated measurements. The generalized cross validation value which is a measure of the model's ability is poured through the box plot. The results show that the use of two smoothing parameters is more optimal than one smoothing parameter. It was seen through a smaller generalized cross validation value on the use of two smoothing parameters. Application of blood sugar level data for patients with two smoothing parameters produced a penalized spline bi-variate predictor regression model with several segments of change patterns. There are five patterns at the time of treatment and blood pressure with the number of smoothing parameters is two, namely 0.39 and 0.73.
Journal of Sciences, Islamic Republic of Iran
University of Tehran
1016-1104
31
v.
2
no.
2020
175
183
https://jsciences.ut.ac.ir/article_76403_aed3c546a5439007a7f74f316ac99e14.pdf
dx.doi.org/10.22059/jsciences.2020.286949.1007435
Persian Abstracts
text
article
2020
eng
Journal of Sciences, Islamic Republic of Iran
University of Tehran
1016-1104
31
v.
2
no.
2020
https://jsciences.ut.ac.ir/article_76404_9c2a21064c108d20ebddeca30cbddbe7.pdf
dx.doi.org/10.22059/jsciences.2020.76404