@article { author = {Azali, Muhammad Azharuddin and Mohamed, Salmah and Harun, Azian and Shamsuddin, Shaharum and Johan, Muhammad Farid}, title = {Optimization of Gene Design, PCR Assembly and Site-Directed Mutagenesis for the Synthesis of Beauveria Bassiana Protease Gene}, journal = {Journal of Sciences, Islamic Republic of Iran}, volume = {32}, number = {1}, pages = {5-14}, year = {2021}, publisher = {University of Tehran}, issn = {1016-1104}, eissn = {2345-6914}, doi = {10.22059/jsciences.2021.306311.1007547}, abstract = {Synthetic gene construction is one of the components of synthetic biology. It can be used for various purposes such as to optimize gene expression. In this study, we proposed six predetermined criteria for designing oligos for the synthesis of the Beauveria bassiana protease gene. These criteria were set up to optimize the cost and to accommodate the oligos assembly. A total of 44 overlapping oligos were designed and synthesized 0.5 µM of oligos mixture was used in assembly PCR together with high fidelity DNA polymerase to produce 1.1 kbp fragment. The gene was visualized by agarose gel electrophoresis before subcloned into pCR™2.1-TOPO. The sequence of the gene was verified by DNA sequencing. Site-directed mutagenesis was performed to repair errors resulted from the gene synthesis. A sharp and distinguished band of the expected size of the protease gene was observed in agarose gel electrophoresis. Errors in the sequence which was detected by DNA sequencing were successfully repaired using our simplified site-directed mutagenesis protocol. The result indicated long DNA sequences (>1 kbp) can be synthesized with less error by using our method. Additionally, this method was easy to perform because it would require minimum optimization to synthesize other genes by following our guidelines.}, keywords = {Gene synthesis,PCR assembly,protease gene,site-directed mutagenesis}, url = {https://jsciences.ut.ac.ir/article_79824.html}, eprint = {} } @article { author = {Ebrahimpour, anita and Riahi Alam, Nader and Khoobi, Mehdi}, title = {The Potential of 5-aminolevulinic Acid as a Contrast Agent in MR Imaging: Challenges and Opportunities}, journal = {Journal of Sciences, Islamic Republic of Iran}, volume = {32}, number = {1}, pages = {15-28}, year = {2021}, publisher = {University of Tehran}, issn = {1016-1104}, eissn = {2345-6914}, doi = {10.22059/jsciences.2020.310908.1007575}, abstract = {Nowadays, 5-aminolevulinic acid (5-ALA) plays an influential role in the detection of malignant tumors as a photodynamic diagnosis tool. Nevertheless, an outlook regarding 5-ALA applicability in magnetic resonance imaging (MRI) has recently emerged. Many studies confirmed the impact of 5-ALA on promoting intracellular Heme synthesis and iron metabolism, which support the capability of 5-ALA in MRI owing to the susceptibility effect of iron. Therefore, concerning the high safety and high affinity of 5-ALA to tumor cells, 5-ALA-based MRI could be an intriguing method for malignant foci identification.}, keywords = {5-aminolevulinic acid,Magnetic resonance imaging,Protoporphyrin IX,Cellular iron metabolism,Transferrin receptor}, url = {https://jsciences.ut.ac.ir/article_79107.html}, eprint = {https://jsciences.ut.ac.ir/article_79107_434a4042fe68f5c5c5f563c6b1007130.pdf} } @article { author = {Zohari, Maryam and Sepahy, Abbass and Amini, Kumarss}, title = {Molecular Cloning and Anti-Cancer Activity of Carotenoid Pigments Isolated from Micrococcus spp. and Rhodotorula spp.}, journal = {Journal of Sciences, Islamic Republic of Iran}, volume = {32}, number = {1}, pages = {29-37}, year = {2021}, publisher = {University of Tehran}, issn = {1016-1104}, eissn = {2345-6914}, doi = {10.22059/jsciences.2020.297577.1007499}, abstract = {Biological sources of pigments receive major attention nowadays because of the stringent rules and regulations applied to chemically synthesized pigments. The aims of this study were isolating carotenoids producing Micrococcus spp. and Rhodotorula spp. from soil sources, optimizing the culture conditions for biomass and carotenoids production, molecular cloning of Crt gene, characterizing yielded pigment, and investigating their anti-cancer activities on human breast cancer cells. Carotenoid producing strains, M. luteus and R. mucilaginosa, were isolated from the soil and sediment samples in Kerman Province, Iran; they were identified using 16srDNA analysis. Optimum conditions for biomass and carotenoids production were determined. Further, Crt gene cloned into pTG19 vector and the effect of carotenoid pigments on MCF-7 breast cancer cell line were evaluated. The optimum growth and carotenoids production by strains were 25°C and pH 7.0 for M. luteus, and 25°C and pH 6.5 for R. mucilaginosa. FTIR and TLC analysis showed high similarity of extracted pigments with carotenoids. The expression levels of Crt genes mRNA found to be approximately 5-fold higher in transformed E. coli in comparison with M. luteus and R. mucilaginosa (p <0.001). MCF-7 cells viability decreased in a concentration and time dependent manner (p <0.05). The doses IC50 against MCF-7 cells for yellow and pink pigments were calculated to be 1426.69 µg/ml (r2=0.95) and 1412.1 µg/ml (r2=0.92), respectively. Microorganisms presented in this study can be used as potential sources of commercial carotenoids production and antitumor metabolites.}, keywords = {Carotenoids,Micrococcus,Rhodotorula,antitumor metabolites}, url = {https://jsciences.ut.ac.ir/article_79109.html}, eprint = {https://jsciences.ut.ac.ir/article_79109_930501e8085482225903e82b5ecb1967.pdf} } @article { author = {Guntarti, Any and Ahda, Mustofa and Nabilla, Hanun and Susanti, Hari}, title = {The Storage Effect against Vitamin C Content in crystal guava (Psidium guajava L.) Juice}, journal = {Journal of Sciences, Islamic Republic of Iran}, volume = {32}, number = {1}, pages = {39-42}, year = {2021}, publisher = {University of Tehran}, issn = {1016-1104}, eissn = {2345-6914}, doi = {10.22059/jsciences.2020.312239.1007585}, abstract = {Guava fruit (Psidium guajava L.) has many varieties, one of which is crystal guava. Crystal guava contains vitamin C which is easily oxidized by temperature. Therefore, this study aimed to determine the levels of vitamin C in crystal guava juice at different storage. Crystal guava samples were obtained from Bogor and then stored at different places and temperatures. Hence, guava fruits were placed in the freezer, refrigerator, and room temperature for 3 days, and all samples were juiced. Based on visual evaluation, guava fruit color is different. Moreover, the quantification method of vitamin C levels in guava juice by HPLC can be carried out with a flow rate of 1 mL/minute using the mobile phase ratio (methanol/water: 5/95, v/v), λmax: 265 nm and C18 as a stationary phase. Retention time of vitamin C in guava is around 2.765 minutes. The effect of storage of vitamin C content in guava juice from fresh samples, and guava stored in the freezer, in the refrigerator, and at room temperature are 3.28 mg/mL, 3.20 mg/mL, 1.65 mg/mL, and 0.82 mg/mL. Therefore, it can be concluded that the best storage of guava fruit is in the freezer because vitamin C levels in crystal guava do not have a significant difference after treatment.}, keywords = {Guava crystal,storage condition,vitamin c}, url = {https://jsciences.ut.ac.ir/article_79104.html}, eprint = {https://jsciences.ut.ac.ir/article_79104_b3fefb22381aff20c522d2be7e5f29c9.pdf} } @article { author = {Derakhsh Ahmadi, Vahid and Rafiei-karahroudi, Z. and Sanatgar, Elham and H. Alizadeh, Babak and Goldasteh, Shila}, title = {Chlorpyrifos-loaded Silver/Polyethylene Glycol/Chitosan Nanocomposite: Improved Termiticidal Activity against Microcerotermes diversus. (Isoptera: Termitidae)}, journal = {Journal of Sciences, Islamic Republic of Iran}, volume = {32}, number = {1}, pages = {43-51}, year = {2021}, publisher = {University of Tehran}, issn = {1016-1104}, eissn = {2345-6914}, doi = {10.22059/jsciences.2020.297633.1007500}, abstract = {Pesticides play an important role in increasing food production by pest control. Most pesticides are less soluble in the aqueous media and need organic solvents for desirable efficacy, causing environmental pollutions. Nanopesticides overcome the problems associated with native pesticides through reduction in the quantity of pesticide and controlling the risks for human and environmental health. In this research, chlorpyrifos (CPS) was loaded in Ag/PEG/Cs nanoparticles (Ag/PEG/Cs/CPS NPs) and then evaluated as a nanopesticide against Microcerotermes diversus Silvestri (Isoptera: Termitidae). The target nanopesticide was characterized by XRD, UV-VIS, FT-IR, and TEM. The results revealed that the NPs were spherical with the medium-sized of 11 nm. The biological activity of the NPs was also estimated in vitro against Microcerotermes diversus. The results confirmed the effectiveness of the nanopesticide at concentrations of 0.05 µl/l (5 ppm) after 48 h against termites and also at a lower concentration of 0.005 µl/l (50 ppm) after 72 h. The nanopesticid could be considered as a promising alternative to the conventional harmful pesticides for controlling termites.}, keywords = {Chlorpyrifos,Nanopesticide,Microcerotermes diversus,nanosilver}, url = {https://jsciences.ut.ac.ir/article_77791.html}, eprint = {https://jsciences.ut.ac.ir/article_77791_fdc7ee6f0aec6339cc1b0e9801fef8e6.pdf} } @article { author = {Naderi, Fereshteh and Menatian, Majid and Behjatmanesh-Ardakani, Reza and zare-mehrjardi, Hamid reza}, title = {DFT Study of Methanol Adsorption on Vacancy and N-Doped Graphene and Comparing Them with Pristine Graphene}, journal = {Journal of Sciences, Islamic Republic of Iran}, volume = {32}, number = {1}, pages = {53-61}, year = {2021}, publisher = {University of Tehran}, issn = {1016-1104}, eissn = {2345-6914}, doi = {10.22059/jsciences.2020.303064.1007529}, abstract = {Abstract In this study, density functional theory was used to investigate the effect of adsorption process and interaction between methanol as a fuel and graphene as a catalyst. Thermodynamic studies in this field have shown that Gibb's free energy is positive in most cases. Therefore, adsorption of methanol on graphene is very low and in the physical mode. Thus, other ways are required to increase adsorption on graphene surface. Changing pristine graphene (PG) to vacancy graphene (VG) or N-doped graphene (NG) can increase absorption, and convert their adsorption into chemical adsorption. Vacancy and N-doped in electronic structure of graphene increase adsorption of methanol to graphene. Increased absorption of VG and NG, in addition to changes in charge transfer causes significant changes in the location of HOMO and LUMO, which was confirmed by adsorption energy, NBO, QTAIM, and DOS.}, keywords = {Adsorption Energy,Density functional theory,Methanol,N-Doped Graphene,Vacancy Graphene}, url = {https://jsciences.ut.ac.ir/article_79108.html}, eprint = {https://jsciences.ut.ac.ir/article_79108_b14de57f6361834c9d29c02a44029230.pdf} } @article { author = {Heydarzadeh, Kobra and Hajialibeigi, Hossein and Gharabeigli, Gholamreza and Verges, Jaume}, title = {Active Deformation Analysis in the Dehdasht Structural Basin Based on Geomorphic Features}, journal = {Journal of Sciences, Islamic Republic of Iran}, volume = {32}, number = {1}, pages = {63-80}, year = {2021}, publisher = {University of Tehran}, issn = {1016-1104}, eissn = {2345-6914}, doi = {10.22059/jsciences.2020.293046.1007465}, abstract = {The Dehdasht Structural Basin, at the boundary of the southern Dezful Embayment and the Izeh Zone, is bordered by large anticlines with Cretaceous to Oligo-Miocene outcrops above hidden basement faults. The drainage system within the Dehdasht Structural Basin and the surrounding is considerably influenced by growth of the Gachsaran diapiric anticlines and salt extrusions and of the boundary anticlines. The Bangestan anticline in the NW of the basin uplifted 655 m since the Maroon River was diverted. Comparing the uplifts recorded in the topography of the large anticlines which are related to the Mountain Front Fault, to their structural amplitudes shows that the Khaviz anticline on the southern Dehdasht Structural Basin has more recent activity than the Bangestan and the Kuh-e-Siah anticlines. Variations of the local base levels along these anticlines with regards to their recent uplift values suggest 30 to 45% contribution of the  thrust faults on their forelimb in the active deformation of the structures bounding the Dehdasht Structural Basin.}, keywords = {Dehdasht Structural Basin,Local base level variations,active deformation,Maroon River,Central Zagros}, url = {https://jsciences.ut.ac.ir/article_77790.html}, eprint = {https://jsciences.ut.ac.ir/article_77790_c9f2401c9d23f805e0d2ddaed50d0a67.pdf} } @article { author = {Ashrafpour, Mehrzad and Ahmadi, Morteza and Jafarpour, Mojtaba}, title = {Analytical Solutions for Entanglement A Superposition of Spin Coherent States with Non-Phase Coherence Parameters}, journal = {Journal of Sciences, Islamic Republic of Iran}, volume = {32}, number = {1}, pages = {81-85}, year = {2021}, publisher = {University of Tehran}, issn = {1016-1104}, eissn = {2345-6914}, doi = {10.22059/jsciences.2020.298214.1007503}, abstract = {In this work, the entanglement of a superposition of bipartite qubit coherent states with non-phased coherent parameters is studied. We use Generalized-concurrence as the measure to quantify the entanglement and drive analytical results in terms of the effective parameters involved. Analyzing the results, we conclude that such states may attain maximum entanglement or no entanglement at all, depending on the choice of the parameters involved.}, keywords = {Entanglement,Generalized concurrence,Spin coherent states,Qubit}, url = {https://jsciences.ut.ac.ir/article_77151.html}, eprint = {https://jsciences.ut.ac.ir/article_77151_d20cf932466b3d81608969dd099bfa78.pdf} } @article { author = {}, title = {Persian Abstract}, journal = {Journal of Sciences, Islamic Republic of Iran}, volume = {32}, number = {1}, pages = {-}, year = {2021}, publisher = {University of Tehran}, issn = {1016-1104}, eissn = {2345-6914}, doi = {}, abstract = {}, keywords = {}, url = {https://jsciences.ut.ac.ir/article_80167.html}, eprint = {https://jsciences.ut.ac.ir/article_80167_7a6f8c976e9891d4708826800ff57ac1.pdf} }