ORIGINAL_ARTICLE
Over Expression of Biologically Active Interferon Beta Using Synthetic Gene in E. coli
In this study, our previously reported novel synthetic gene encoding 166 residues of interferon-? was used for an efficient expression of IFN-?. The synthetic gene was cloned into pET21a expression vector and transferred into E. coli. Recombinant protein was over-expressed in the E. coli. Identity of the recombinant protein was confirmed by western blot analysis. The recombinant protein was biologically active as evaluated by inhibition of cytopathic effect (CPE) formation of Vesicular stomatitis virus (VSV) on the HeLa cells. The effect of three factors including inducer concentration, induction time based on optical density of the culture and induction duration on the expression of rIFN-? was investigated by Taguchi method. Analysis of variance presented that IPTG of 0.5 mM and induction duration of 4 h and induction time of OD600=1 had more effect on IFN-? production. Recombinant IFN-? expression with the above condition yielded 28% of the total E. coli proteins.
https://jsciences.ut.ac.ir/article_31893_d1b8bbbfc65547a2d1476ff0842c07e7.pdf
2008-09-01
ORIGINAL_ARTICLE
Analysis of Exon 19 and 39 of ATM Gene in Brain Tumors; Considering the P53 Accumulation
Many studies have been focused on cytogenetic and molecular genetic defects in brain tumors; therefore the role of ATM as a tumor suppressor gene in these tumors is poorly considered. In this study mutation analysis of exon 19 and 39 of ATM gene and P53 accumulation were investigated by PCR-SSCP, sequencing, and flow cytometry . Four polymorphisms including D1853N, IVS 38-8 T?C, F858L, P872T were reported for the first time in brain tumors other than medulloblastoma. Expression of P53 could be detected in more than 10% of cells in patients affected with meningioma and 4.08% and 3.46% of cells inastrocytoma and chordoma respectively. The present findings could confirm the importance of ATM gene alterations in tumor genesis of brain tumors and further investigation is essential.
https://jsciences.ut.ac.ir/article_31894_c46b217a74acf05509eee3d5a1f96334.pdf
2008-09-01
ORIGINAL_ARTICLE
Synthesis and Pharmacological Evaluation of Some Novel Isatin Derivatives for Antimicrobial Activity
In the present work, a series of new 5-substituted-3-(4-arylimino)-1-[5-mercapto(1,3,4-oxadiazolyl)]-methyl-indol-2-one (4a-g) have been synthesized by heterocyclization of 5-substituted-3-(4-arylimino)-2-oxo-1-indole acetylhyrazide (3a-g) on treatment with CS2 in ethanolic KOH. The compound 4a was characterized by its elemental analysis, IR, 1HNMR and Mass Spectroscopy. The synthesized compounds (4a-g) were evaluated for in vitro antibacterial activity and antifungal activity against various strains of bacteria and fungi.
https://jsciences.ut.ac.ir/article_31895_981b4487122b372fdfd27ff42d00fa12.pdf
2008-09-01
ORIGINAL_ARTICLE
Post-Collisional Plio-Pleistocene Adakitic Volcanism in Centeral Iranian Volcanic Belt:Geochemical
In the Central IranianVolcanic Belt (CIVB), north-west of Shahre-Babak, in the area of Javazm, Dehaj and khabr, about 60 subvolcanic porphyritic dacitic to rhyodacitic domes (1-10 km2) are intruded into a variety of rock sequences from Mesozoic to Early Miocene in age. These rocks are a part of Dehaj-Sardoieh belt. The CIVB contains intrusive and extrusive rocks of Cretaceous-Quaternary age. Geochemical data indicate that the subalkalic dacitic to rhyodacitic rocks have an adakitic composition with Na2o/K2o (1.8-3.16), high Sr (584-1750 ppm), Mg # = (0.18-0.57) and low Y (7-10 ppm), low Yb (0.65-1.29 ppm), and low HREE. Fractionated REE patterns, (Ce/Yb)N = 10-27, absence of negative Eu anomal, low content of Y, Nb, Ti, and high Sr/Y (74-265) and (Ce/Yb)N ratios suggest that the source was probably hydrous garnet-amphibolite or amphibole-eclogite, possibly generated during subduction of the Neo-Tethyan oceanic slab beneath the Central Iran microplate.The adakitic volcanism was followed by eruption of alkaline magmatism in this area. Slab melting occurred after cessation of subduction, possibility from the collision. Transtensional tectonics accompanied by a locally extensional stress regime account for magma genesis and ascent.
https://jsciences.ut.ac.ir/article_31896_3d5550b30b2590c75543469f305410a2.pdf
2008-09-01
ORIGINAL_ARTICLE
Garnet-Biotite Chemistry for Thermometry of Staurolite Schist from South of Mashhad, NE Iran
Mashhad Metamorphic Complex (MMC) with metamorphic, granitic intrusions and ophiolite units is situated in the suture zone of Paleotethys in NE Iran. Triassic staurolite-garnet schist of Khalaj represents the highest grade of metamorphism in MMC. In this study EPMA analyses of garnet and biotite used to determine the maximum temperature for regional metamorphism. The pattern of zoning in garnet also used to describe the exhumation history of the area during metamorphic events. Maximum temperature for staurolite garnet schist of MMC calculated with garnet-biotite thermometer is ~618°C. The quantitative analysis of major elements along core to rim profiles obtained for garnet porphyroblast show the presence of growth zoning. Mg and Fe increase slightly outwards, while Mn decreases from core to the rim. Preserved garnet growth zoning in temperature above 600°C, indicates that staurolite-garnet schists of MMC on the margin of Paleotethys in NE Iran were rapidly heated and cooled. However, growth zoning is not well preserved in the rim of garnet grain. It could be an evidence for the presence of later retrograde metamorphic phase which influenced regional metamorphic schists in MMC.
https://jsciences.ut.ac.ir/article_31897_ebd92d6381d2aab1151e6a2f6e3ca26c.pdf
2008-09-01
ORIGINAL_ARTICLE
Gold Distribution in Porphyry Copper Deposits of Kerman Region, Southeastern Iran
https://jsciences.ut.ac.ir/article_31898_d85486b4c0968032c431d13c3a137f20.pdf
2008-09-01
ORIGINAL_ARTICLE
Laws of Large Numbers for Random Linear
The computational solution of large scale linear programming problems contains various difficulties. One of the difficulties is to ensure numerical stability. There is another difficulty of a different nature, namely the original data, contains errors as well. In this paper, we show that the effect of the random errors in the original data has a diminishing tendency for the optimal value as the number of constraints and the number of variables increase. The laws of large numbers in probability theory are mathematical formulations for indicating the slowing-down tendency of the effect of random errors in the data. This paper was inspired by the paper of Prekopa [3]. Prekopa [3] proved both weak and strong laws of large numbers for the random linear programs in independence setting. We obtain laws of large numbers under negatively associated dependence for random linear programs and we extend Prekopa's results [3] to the case of negatively associated random variables.
https://jsciences.ut.ac.ir/article_31899_31d921ba60dd53ba60010334afc06b9f.pdf
2008-09-01
ORIGINAL_ARTICLE
A Note on Artinianess of Certain Generalized
Let ?: R0?R be a ring homomorphism and suppose that a and a0, respectively, are ideals of R and R0 such that is an Artinian ring. Let M and N be two finitely generated R-modules and suppose that (R0,m0) is a local ring. In this note we prove that the R-modules and are Artinian for all integers i and j, whenever and . Also we will show that if a is principal, then the R-modules and are Artinian, for all integers i and j. Moreover, we will show that if is the largest integer i such that is not Artinian, then the R-modules and are Artinian, for and all . Also as a consequence of this result we get that the R-modules and are artinian, for j=d, d?1, in which is the cohomological dimension of M and N with respect to a. Our results recover the corresponding known ones.
https://jsciences.ut.ac.ir/article_31900_2baca006c34e6f98743d9cab322edc02.pdf
2008-09-01
ORIGINAL_ARTICLE
CFD-Calculation of Fluid Flow in a
An accurate description of the fluid flow and heat transfer within a Pressurized Water Reactor (PWR), for the safety analysis and reactor performance is always desirable. In this paper a mathematical model of the fundamental physical phenomena which are associated to a typical PWR is presented. The mathematical model governs the fluid dynamics in the reactor. Using commercial software CFX, a computational fluid dynamics (CFD) code, a three-dimensional flow distribution in the downcomer and the lower plenum of the reactor was also calculated and a valuable analysis of the reactor performance is given. Due to computational limits, simplifications of the core, downcomer and the lower plenum of the reactor are introduced. Nevertheless, it has been shown that computational fluid dynamics and in particular appropriate usage of CFX software improves our understanding of fluid flow distribution, velocity distribution and heat transfer in different parts of the reactor pressure vessel, in particular, in the downcomer and the lower plenum.
https://jsciences.ut.ac.ir/article_31901_b3c0fa9ddcfec8b545f2eccc2017f6a9.pdf
2008-09-01