University of Tehran
Journal of Sciences, Islamic Republic of Iran
1016-1104
2345-6914
32
1
2021
03
01
Optimization of Gene Design, PCR Assembly and Site-Directed Mutagenesis for the Synthesis of Beauveria Bassiana Protease Gene
5
14
EN
Muhammad Azharuddin
Azali
0000-0001-7019-6991
1 Department of Haematology, School of Medical Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia.
2 School of Agriculture Science and Biotechnology, Faculty of Bioresources and Food Industry, Universiti Sultan Zainal Abidin, 22200 Besut, Terengganu, Malaysia
azharuddinazali@unisza.edu.my
Salmah
Mohamed
0000-0001-9194-3925
2 School of Agriculture Science and Biotechnology, Faculty of Bioresources and Food Industry, Universiti Sultan Zainal Abidin, 22200 Besut, Terengganu, Malaysia.
salmahmohamed@unisza.edu.my
Azian
Harun
0000-0002-0426-2855
3 Department of Microbiology & Parasitology, School of Medical Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia.
azian@usm.my
Shaharum
Shamsuddin
0000-0001-9997-7740
4 School of Health Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia.
5 Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia
shaharum1@usm.my
Muhammad Farid
Johan
0000-0001-6344-0220
1Department of Haematology, School of Medical Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia
faridjohan@usm.my
10.22059/jsciences.2021.306311.1007547
Synthetic gene construction is one of the components of synthetic biology. It can be used for various purposes such as to optimize gene expression. In this study, we proposed six predetermined criteria for designing oligos for the synthesis of the <em>Beauveria</em> bassiana protease gene. These criteria were set up to optimize the cost and to accommodate the oligos assembly. A total of 44 overlapping oligos were designed and synthesized 0.5 µM of oligos mixture was used in assembly PCR together with high fidelity DNA polymerase to produce 1.1 kbp fragment. The gene was visualized by agarose gel electrophoresis before subcloned into pCR™2.1-TOPO. The sequence of the gene was verified by DNA sequencing. Site-directed mutagenesis was performed to repair errors resulted from the gene synthesis. A sharp and distinguished band of the expected size of the protease gene was observed in agarose gel electrophoresis. Errors in the sequence which was detected by DNA sequencing were successfully repaired using our simplified site-directed mutagenesis protocol. The result indicated long DNA sequences (>1 kbp) can be synthesized with less error by using our method. Additionally, this method was easy to perform because it would require minimum optimization to synthesize other genes by following our guidelines.
Gene synthesis,PCR assembly,protease gene,site-directed mutagenesis
https://jsciences.ut.ac.ir/article_79824.html
https://jsciences.ut.ac.ir/article_79824_d41d8cd98f00b204e9800998ecf8427e.pdf
University of Tehran
Journal of Sciences, Islamic Republic of Iran
1016-1104
2345-6914
32
1
2021
03
01
The Potential of 5-aminolevulinic Acid as a Contrast Agent in MR Imaging: Challenges and Opportunities
15
28
EN
anita
Ebrahimpour
Department of Medical Physics and Biomedical Engineering, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Islamic Republic of Iran.
anitaebrahimpor@gmail.com
Nader
Riahi Alam
Department of Medical Physics and Biomedical Engineering, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Islamic Republic of Iran.
riahialam@gmail.com
Mehdi
Khoobi
The Institute of Pharmaceutical Sciences (TIPS), Tehran University of Medical Sciences, Tehran, Iran
m-khoobi@tums.ac.ir
10.22059/jsciences.2020.310908.1007575
Nowadays, 5-aminolevulinic acid (5-ALA) plays an influential role in the detection of malignant tumors as a photodynamic diagnosis tool. Nevertheless, an outlook regarding 5-ALA applicability in magnetic resonance imaging (MRI) has recently emerged. Many studies confirmed the impact of 5-ALA on promoting intracellular Heme synthesis and iron metabolism, which support the capability of 5-ALA in MRI owing to the susceptibility effect of iron. Therefore, concerning the high safety and high affinity of 5-ALA to tumor cells, 5-ALA-based MRI could be an intriguing method for malignant foci identification.
5-aminolevulinic acid,Magnetic resonance imaging,Protoporphyrin IX,Cellular iron metabolism,Transferrin receptor
https://jsciences.ut.ac.ir/article_79107.html
https://jsciences.ut.ac.ir/article_79107_434a4042fe68f5c5c5f563c6b1007130.pdf
University of Tehran
Journal of Sciences, Islamic Republic of Iran
1016-1104
2345-6914
32
1
2021
03
01
Molecular Cloning and Anti-Cancer Activity of Carotenoid Pigments Isolated from Micrococcus spp. and Rhodotorula spp.
29
37
EN
Maryam
Zohari
Ph.D. Student, Department of biology, Faculty of Basic Science, Science and Research Branch, Islamic Azad University, Tehran, Iran
Abbass
Akhavan
Sepahy
0000000241128962
Department of Microbiology, Faculty of Basic Siences, North Tehran Branch, Islamic Azad University, Tehran, Iran
akhavansepahy@gmail.com
Kumarss
Amini
Department of Microbiology, Faculty of Basic Science, Saveh Branch, Islamic Azad University, Markazi, Iran
dr_kumarss_amini@yahoo.com
10.22059/jsciences.2020.297577.1007499
Biological sources of pigments receive major attention nowadays because of the stringent rules and regulations applied to chemically synthesized pigments. The aims of this study were isolating carotenoids producing <em>Micrococcus spp. and Rhodotorula spp.</em> from soil sources, optimizing the culture conditions for biomass and carotenoids production, molecular cloning of Crt gene, characterizing yielded pigment, and investigating their anti-cancer activities on human breast cancer cells. Carotenoid producing strains, <em>M. luteus </em>and<em> R. mucilaginosa,</em> were isolated from the soil and sediment samples in Kerman Province, Iran; they were identified using 16srDNA analysis. Optimum conditions for biomass and carotenoids production were determined. Further, Crt gene cloned into pTG19 vector and the effect of carotenoid pigments on MCF-7 breast cancer cell line were evaluated. The optimum growth and carotenoids production by strains were 25°C and pH 7.0 for <em>M. luteus</em>, and 25°C and pH 6.5 for <em>R. mucilaginosa</em>. FTIR and TLC analysis showed high similarity of extracted pigments with carotenoids. The expression levels of Crt genes mRNA found to be approximately 5-fold higher in transformed <em>E. coli</em> in comparison with <em>M. luteus</em> and <em>R. mucilaginosa</em> (p <0.001). MCF-7 cells viability decreased in a concentration and time dependent manner (p <0.05). The doses IC<sub>50</sub> against MCF-7 cells for yellow and pink pigments were calculated to be 1426.69 µg/ml (r<sup>2</sup>=0.95) and 1412.1 µg/ml (r<sup>2</sup>=0.92), respectively. Microorganisms presented in this study can be used as potential sources of commercial carotenoids production and antitumor metabolites.
Carotenoids,Micrococcus,Rhodotorula,antitumor metabolites
https://jsciences.ut.ac.ir/article_79109.html
https://jsciences.ut.ac.ir/article_79109_930501e8085482225903e82b5ecb1967.pdf
University of Tehran
Journal of Sciences, Islamic Republic of Iran
1016-1104
2345-6914
32
1
2021
03
01
The Storage Effect against Vitamin C Content in crystal guava (Psidium guajava L.) Juice
39
42
EN
Any
Guntarti
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Ahmad Dahlan, Yogyakarta, Indonesia
any_guntarti@yahoo.co.id
Mustofa
Ahda
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Ahmad Dahlan, Yogyakarta, Indonesia
mustofa_ahda@yahoo.com
Hanun
Nabilla
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Ahmad Dahlan, Yogyakarta, Indonesia
Hari
Susanti
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Ahmad Dahlan, Yogyakarta, Indonesia
mustofa.ahda.uad@gmail.com
10.22059/jsciences.2020.312239.1007585
Guava fruit (<em>Psidium guajava</em> L.) has many varieties, one of which is crystal guava. Crystal guava contains vitamin C which is easily oxidized by temperature. Therefore, this study aimed to determine the levels of vitamin C in crystal guava juice at different storage. Crystal guava samples were obtained from Bogor and then stored at different places and temperatures. Hence, guava fruits were placed in the freezer, refrigerator, and room temperature for 3 days, and all samples were juiced. Based on visual evaluation, guava fruit color is different. Moreover, the quantification method of vitamin C levels in guava juice by HPLC can be carried out with a flow rate of 1 mL/minute using the mobile phase ratio (methanol/water: 5/95, v/v), λmax: 265 nm and C18 as a stationary phase. Retention time of vitamin C in guava is around 2.765 minutes. The effect of storage of vitamin C content in guava juice from fresh samples, and guava stored in the freezer, in the refrigerator, and at room temperature are 3.28 mg/mL, 3.20 mg/mL, 1.65 mg/mL, and 0.82 mg/mL. Therefore, it can be concluded that the best storage of guava fruit is in the freezer because vitamin C levels in crystal guava do not have a significant difference after treatment.
Guava crystal,storage condition,vitamin c
https://jsciences.ut.ac.ir/article_79104.html
https://jsciences.ut.ac.ir/article_79104_b3fefb22381aff20c522d2be7e5f29c9.pdf
University of Tehran
Journal of Sciences, Islamic Republic of Iran
1016-1104
2345-6914
32
1
2021
03
01
Chlorpyrifos-loaded Silver/Polyethylene Glycol/Chitosan Nanocomposite: Improved Termiticidal Activity against Microcerotermes diversus. (Isoptera: Termitidae)
43
51
EN
Vahid
Derakhsh Ahmadi
Department of Entomology, Arak Branch, Islamic Azad University, Arak, Islamic Republic of Iran
versasanaatsabz@yahoo.com
Z.
Rafiei-karahroudi
Department of Entomology, Arak Branch, Islamic Azad University, Arak, Islamic Republic of Iran
Elham
Sanatgar
Department of Entomology, Arak Branch, Islamic Azad University, Arak, Islamic Republic of Iran
Babak
H. Alizadeh
Department of Pesticides, Iranian Research Institute of Plant Protection, Agricultural Research, Education and Extension Organization (AREEO), Islamic Republic of Iran
alizadehbh18@gmail.com
Shila
Goldasteh
Department of Entomology, Arak Branch, Islamic Azad University, Arak, Islamic Republic of Iran
shilagoldasteh@yahoo.com
10.22059/jsciences.2020.297633.1007500
Pesticides play an important role in increasing food production by pest control. Most pesticides are less soluble in the aqueous media and need organic solvents for desirable efficacy, causing environmental pollutions. Nanopesticides overcome the problems associated with native pesticides through reduction in the quantity of pesticide and controlling the risks for human and environmental health. In this research, chlorpyrifos (CPS) was loaded in Ag/PEG/Cs nanoparticles (Ag/PEG/Cs/CPS NPs) and then evaluated as a nanopesticide against <em>Microcerotermes diversus</em> Silvestri (Isoptera: Termitidae). The target nanopesticide was characterized by XRD, UV-VIS, FT-IR, and TEM. The results revealed that the NPs were spherical with the medium-sized of 11 nm. The biological activity of the NPs was also estimated <em>in vitro</em> against <em>Microcerotermes diversus</em>. The results confirmed the effectiveness of the nanopesticide at concentrations of 0.05 µl/l (5 ppm) after 48 h against termites and also at a lower concentration of 0.005 µl/l (50 ppm) after 72 h. The nanopesticid could be considered as a promising alternative to the conventional harmful pesticides for controlling termites.
Chlorpyrifos,Nanopesticide,Microcerotermes diversus,nanosilver
https://jsciences.ut.ac.ir/article_77791.html
https://jsciences.ut.ac.ir/article_77791_fdc7ee6f0aec6339cc1b0e9801fef8e6.pdf
University of Tehran
Journal of Sciences, Islamic Republic of Iran
1016-1104
2345-6914
32
1
2021
03
01
DFT Study of Methanol Adsorption on Vacancy and N-Doped Graphene and Comparing Them with Pristine Graphene
53
61
EN
Fereshteh
Naderi
0000-0001-7995-0495
Department of Chemistry, Shahr-e Qods branch, Islamic Azad University, Tehran, Iran
fnaderi1@gmail.com
Majid
Menatian
Department of chemistry, Payame Noor University, P.O.Box:19395-3697 Tehran, Iran
am.menatian@gmail.com
Reza
Behjatmanesh-Ardakani
0000-0001-9163-9333
Department of chemistry, Payame Noor University, P.O.Box:19395-3697 Tehran, Iran
reza_b_m_a@yahoo.com
Hamid reza
zare-mehrjardi
0000-0001-9059-7530
Department of chemistry, Payame Noor University, P.O.Box:19395-3697 Tehran, Iran
hr_zare@pnu.ac.ir
10.22059/jsciences.2020.303064.1007529
Abstract
In this study, density functional theory was used to investigate the effect of adsorption process and interaction between methanol as a fuel and graphene as a catalyst. Thermodynamic studies in this field have shown that Gibb's free energy is positive in most cases. Therefore, adsorption of methanol on graphene is very low and in the physical mode. Thus, other ways are required to increase adsorption on graphene surface. Changing pristine graphene (PG) to vacancy graphene (VG) or N-doped graphene (NG) can increase absorption, and convert their adsorption into chemical adsorption. Vacancy and N-doped in electronic structure of graphene increase adsorption of methanol to graphene. Increased absorption of VG and NG, in addition to changes in charge transfer causes significant changes in the location of HOMO and LUMO, which was confirmed by adsorption energy, NBO, QTAIM, and DOS.
Adsorption Energy,Density functional theory,Methanol,N-Doped Graphene,Vacancy Graphene
https://jsciences.ut.ac.ir/article_79108.html
https://jsciences.ut.ac.ir/article_79108_b14de57f6361834c9d29c02a44029230.pdf
University of Tehran
Journal of Sciences, Islamic Republic of Iran
1016-1104
2345-6914
32
1
2021
03
01
Active Deformation Analysis in the Dehdasht Structural Basin Based on Geomorphic Features
63
80
EN
Kobra
Heydarzadeh
Department of Sedimentary Basins and Petroleum, Facaulty of Earth Sciences, Shahid Beheshti University
Hossein
Hajialibeigi
Department of Sedimentary Basins and Petroleum, Facaulty of Earth Sciences,Shahid Beheshti University
h-alibeigi@sbu.ac.ir
Gholamreza
Gharabeigli
National Iranian Oil Company, exploration directorate, Tehran
g.gharabeigli1975@gmail.com
Jaume
Verges
Earth Science instute of Jaume Almera, Barcelona, Spain
10.22059/jsciences.2020.293046.1007465
The Dehdasht Structural Basin, at the boundary of the southern Dezful Embayment and the Izeh Zone, is bordered by large anticlines with Cretaceous to Oligo-Miocene outcrops above hidden basement faults. The drainage system within the Dehdasht Structural Basin and the surrounding is considerably influenced by growth of the Gachsaran diapiric anticlines and salt extrusions and of the boundary anticlines. The Bangestan anticline in the NW of the basin uplifted 655 m since the Maroon River was diverted. Comparing the uplifts recorded in the topography of the large anticlines which are related to the Mountain Front Fault, to their structural amplitudes shows that the Khaviz anticline on the southern Dehdasht Structural Basin has more recent activity than the Bangestan and the Kuh-e-Siah anticlines. Variations of the local base levels along these anticlines with regards to their recent uplift values suggest 30 to 45% contribution of the thrust faults on their forelimb in the active deformation of the structures bounding the Dehdasht Structural Basin.
Dehdasht Structural Basin,Local base level variations,active deformation,Maroon River,Central Zagros
https://jsciences.ut.ac.ir/article_77790.html
https://jsciences.ut.ac.ir/article_77790_c9f2401c9d23f805e0d2ddaed50d0a67.pdf
University of Tehran
Journal of Sciences, Islamic Republic of Iran
1016-1104
2345-6914
32
1
2021
03
01
Analytical Solutions for Entanglement A Superposition of Spin Coherent States with Non-Phase Coherence Parameters
81
85
EN
Mehrzad
Ashrafpour
Department of Physics, Faculty of Science , Shahid Chamran University of Ahvaz, Ahvaz, Iran
mehrzadashrafpour@yahoo.com
Morteza
Ahmadi
Department of Physics, Faculty of Science , Shahid Chamran University of Ahvaz, Iran
mortezaahmadi1990@yahoo.com
Mojtaba
Jafarpour
Department of Physics, Faculty of Science , Shahid Chamran University of Ahvaz, Iran
mojtaba_jafarpour@hotmail.com
10.22059/jsciences.2020.298214.1007503
In this work, the entanglement of a superposition of bipartite qubit coherent states with non-phased coherent parameters is studied. We use Generalized-concurrence as the measure to quantify the entanglement and drive analytical results in terms of the effective parameters involved. Analyzing the results, we conclude that such states may attain maximum entanglement or no entanglement at all, depending on the choice of the parameters involved.
Entanglement,Generalized concurrence,Spin coherent states,Qubit
https://jsciences.ut.ac.ir/article_77151.html
https://jsciences.ut.ac.ir/article_77151_d20cf932466b3d81608969dd099bfa78.pdf
University of Tehran
Journal of Sciences, Islamic Republic of Iran
1016-1104
2345-6914
32
1
2021
03
01
Persian Abstract
EN
https://jsciences.ut.ac.ir/article_80167.html
https://jsciences.ut.ac.ir/article_80167_7a6f8c976e9891d4708826800ff57ac1.pdf