University of Tehran
Journal of Sciences, Islamic Republic of Iran
1016-1104
2345-6914
6
3
1995
08
01
PURIFICATION AND PROPERTIES OF AN
EXTRACELLULAR PROTEASE PRODUCED BY
PENICIUIUM EXPANSUM
EN
Penicilliurn expamum grown in a medium with rice husk as a carbon source
produced an extracellular protease. The protease enzyme was isolated from culture
broth by fractionation with acetone and column chromatography on Sephadex G- 100
and DEAE A-50. The protease enzyme was purified about 17.47 fold, with a recovery
of 14%. The purified protease was homogenous on SDS polyacrylarnide disc gel
electrophoresis and molecular weight was calculated to be 28000. The optimum
activity of protease was found at pH 7.5 and temperature 30°C. The enzyme activity
was enhanced on addition of Ca , Zn , Co and Mn whereas inhibition was
observed in the presence of EDTA, Hg and Ag . Addition of cysteine and 2-
mercaptoethanol did not produce any significant effect on protease activity
https://jsciences.ut.ac.ir/article_31331.html
https://jsciences.ut.ac.ir/article_31331_7ea250ae4aaa03b6b1f2d17f26ff5277.pdf
University of Tehran
Journal of Sciences, Islamic Republic of Iran
1016-1104
2345-6914
6
3
1995
08
01
POLYPHENOL OXIDASE ACTIVITY AND
DIFFERENTIATION OF RHIZOMORPH IN
ARMILLARIA MELLEA (VAML. EX. FR.) KARST
EN
Polyphenol oxidase (PPO) activity varied in differentiation from hyphae to
rhizomorph and croute in A. mellea. In undifferentiated hyphae there was not
any PPO activity, while croute showed the highest activity. Electrophoresis in
the presence of SDS allows the detection of intensive enzyme forms of PPO,
and can also convert slower migrating enzyme forms to faster ones. Some
fluorescent compounds, as intense activators of PPO, show an intense band of
PPO in electrophoresis but not in the migrating band
https://jsciences.ut.ac.ir/article_31332.html
https://jsciences.ut.ac.ir/article_31332_33fcf9306a7ff8688f5a6975fdf53c92.pdf
University of Tehran
Journal of Sciences, Islamic Republic of Iran
1016-1104
2345-6914
6
3
1995
08
01
THE EFFECTS OF COPPER ON CYTOCHROMES
BIOSYNTHESIS, CELL DIVISION IWD
MUTAGENESIS IN PARACOCCUS
DENITRIFICANS
EN
https://jsciences.ut.ac.ir/article_31333.html
https://jsciences.ut.ac.ir/article_31333_645b9924c67b3b14eb3ad24a9684749a.pdf
University of Tehran
Journal of Sciences, Islamic Republic of Iran
1016-1104
2345-6914
6
3
1995
08
01
INTERACTION OF DOXORUBICIN WITH
DNA-HMG1 COMPLEX
EN
In this study, the interaction of the anthracycline antibiotic doxorubicin
with DNA-HMG 1 complex was investigated employing UV/VIS spectroscopy,
thermal denaturation and DNA cellulose chromatography techniques. The
results indicated that the binding of doxorubicin to the protein reduces its Tm
in a dose dependent manner. Although doxorubicin protects free DNA
against thermal denaturation in the presence of HMG 1, thermal melts represent
biphasic profile corresponding to DNA- drug, HMG1- drug and free
components. Interaction of antibiotic with DNA - HMGl complex using
DNA cellulose columns also implies that the drug binds strongly to singlestranded
DNA-HMG 1 complex rather than to double- stranded DNA-HMG 1
columns. The results suggest destabilization of HMGl protein by drug action
thereby preventing DNA-HMG 1 complex formation
https://jsciences.ut.ac.ir/article_31334.html
https://jsciences.ut.ac.ir/article_31334_d8baabdbd0ee99b0d7fa5b84008f7955.pdf
University of Tehran
Journal of Sciences, Islamic Republic of Iran
1016-1104
2345-6914
6
3
1995
08
01
PREPARATION AND CHARACTERIZATION OF
[Ni (MAP) ] ;
H- MAP = 2- (METHYLAMINO) PYRIDINE
H. Aghabozorg* and H. H. Monfared
EN
We have recently synthesized [Ni (MAP) ] complex [H-MAP= 2- (methylamino)
pyridine]. 2- (Methy1amino)pyridine reacts with MeLi in THF and generates the
lithium salt (Li-MAP). Further reaction of NiCl with two equivalent of this ligand in
THF leads to the formation of a dark red solution. Analytical data, molecular weight,
and magnetic susceptibility of this complex confirm the formula [Ni (MAP) ] .
https://jsciences.ut.ac.ir/article_31335.html
https://jsciences.ut.ac.ir/article_31335_419960dbb76cf621d5f97bb7fa293667.pdf
University of Tehran
Journal of Sciences, Islamic Republic of Iran
1016-1104
2345-6914
6
3
1995
08
01
SYNTHESIS OF A SPECIAL TYPE OF
ZEOLITE FROM ANALCIME FAMILY WITH
TRIETHANOLAMINE AND
ITS CHARACTERIZATION WITH
XRD, FTIR AND SEM
EN
A type of zeolite from the analcime family was prepared by using
triethanolamine, silica gel and sodium aluminate. The average particle size was
around 24 µm. FTIR, XRD and SEM results approved the type of zeolite which
was formed
https://jsciences.ut.ac.ir/article_31336.html
https://jsciences.ut.ac.ir/article_31336_c1fd41a580c402eedd396556058673e8.pdf
University of Tehran
Journal of Sciences, Islamic Republic of Iran
1016-1104
2345-6914
6
3
1995
08
01
SYNTHESIS OF 4,4'-BIS-PYRIMIDINES AND
SOME RELATED BIS-FUSED PYRIMIDINES
EN
2,2'-Disubstitutedamino-4-4'-bis-pyrimidines (3a-m) have been prepared by
linking 2,4-dichloro-6-methyl-5-nitropyrimidine (1) with several diamines and
subsequent reaction with suitable amines. Some of these compounds (3c and 3j)
which were reduced catalitically, on treatment with nitrous acid, urea in
pyridine, and boiling thionyl chloride gave the corresponding bistriazolopyrimidin
(6), bis-purines (7), and bis-isothiazolopyrimidines (8). Two
bis-imidazo[l,2-a] pyrimidines (9 and 10) have also been prepared
https://jsciences.ut.ac.ir/article_31337.html
https://jsciences.ut.ac.ir/article_31337_ecd0707c149a2fbec692e037c82c15a1.pdf
University of Tehran
Journal of Sciences, Islamic Republic of Iran
1016-1104
2345-6914
6
3
1995
08
01
LITHIUM PERCHLORATE MEDIATED
AMINATION REACTION OF ALDEHYDES:
A NOVEL METHOD FOR SYNTHESIS OF N,
N-DIALKYL-p-SILYLATED AMINES*
EN
P-Silylated N,N-dialkyl amines (5) were easily synthesized in good to
excellent yield from aldehydes (1), trimethylsilyldialkylamines (2), and
trimethylsilylmethylmagnesium chloride (4) in the presence of 5 M lithium
perchlorate solution in diethyl ether. The reaction Of (2) with aldehyde
proceeded smoothly in the LiCI0 , to give the imimum salt (3). Addition of
trimethylsilylmethylrnagnesium chloride (4) gave the desired product
https://jsciences.ut.ac.ir/article_31338.html
https://jsciences.ut.ac.ir/article_31338_608334d9d8a3c8cdef9532da9b190fb6.pdf
University of Tehran
Journal of Sciences, Islamic Republic of Iran
1016-1104
2345-6914
6
3
1995
08
01
SELENIUM HETEROCYCLE XL II [1].
SYNTHESES OF SUBSTITUTED THIAZOLO,
SELENAZOLO AND
1,2,3-SELENADIAZOLOINDOLES
EN
Reaction of compound 1 with thiobenzarnide or substituted selenobenzamides
afforded substituted pyrrolobenzothiazole or selenazoles (2) respectively. Selenium
dioxide oxidation of the semicarbazone 4 with selenium dioxide gave 6-
benzenesulfonyl- 4,5-dihydro-pynolo [3,2-el benzoselenadiazole (5).
https://jsciences.ut.ac.ir/article_31339.html
https://jsciences.ut.ac.ir/article_31339_a47da1f9300383f005d9c9a5d5b66708.pdf
University of Tehran
Journal of Sciences, Islamic Republic of Iran
1016-1104
2345-6914
6
3
1995
08
01
STATISTICAL-MECHANICAL CALCULATION
OF THERMODYNAMIC PROPERTIES OF
ALBITE USING A TWO-DIMENSIONAL MODEL
EN
https://jsciences.ut.ac.ir/article_31340.html
https://jsciences.ut.ac.ir/article_31340_d41b2123aaec8266eac60bd5ed75729f.pdf
University of Tehran
Journal of Sciences, Islamic Republic of Iran
1016-1104
2345-6914
6
3
1995
08
01
AXIAL FLOW OF CASSON PLASTIC MODEL
IN ANNULUS WITH A ROTATING
INNER CYLINDER
EN
https://jsciences.ut.ac.ir/article_31341.html
https://jsciences.ut.ac.ir/article_31341_9b285dd57ae9fd2ae52e42359bdc3999.pdf
University of Tehran
Journal of Sciences, Islamic Republic of Iran
1016-1104
2345-6914
6
3
1995
08
01
HELICITY AND PLANAR AMPLITUDES IN
PION-PROTON SCATTERING AT 6.0 GeV/c
EN
In addition to optimal conditions, invariant laws of Lorentz, parity and time
reversal are imposed to find the relation between observables (spin rotation
parameters) and bilinear combination of helicity amplitudes in pion-proton
elastic scattering at 6.0 GeV/c. By normalizing the differential cross-section to
unity, the magnitudes of helicity amplitudes and the angle between them are
determined. Planar amplitudes are found in terms of the helicity amplitudes and
the angle between momentum vector and quantization direction in the reaction
plane.
https://jsciences.ut.ac.ir/article_31342.html
https://jsciences.ut.ac.ir/article_31342_4df098407f89202d9b3724cb66dedc5f.pdf