TY - JOUR ID - 31609 TI - Purification and Characterization of an Antibacterial, Antifungal and Non Hemolytic Peptide from Rana Ridibunda JO - Journal of Sciences, Islamic Republic of Iran JA - JSCIENCES LA - en SN - 1016-1104 Y1 - 2004 PY - 2004 VL - 15 IS - 4 SP - EP - DO - N2 - Amphibians have a large variety of antimicrobial peptides that serve as natural innate barriers limiting microbial infection or, in some instances, act as an integral component in response to inflammation or microbial infection. A novel peptide with antibacterial effects and without hemolytic activity was purified from skin secretions of Rana ridibunda by multisteps cation-exchange FPLC, reversed-phase HPLC and was called Ridibundin 1. Circular dichroism spectra revealed that this peptide strongly prefers to form an amphipathic ?-helical structure in the presence of 50% trifluoroethanol. Acting as wide-spectrum microbicides against a variety of bacteria, Ridibundin 1 also shows no hemolytic activity on erythrocyte. These properties reveal its unique characteristics and potential therapeutic application UR - https://jsciences.ut.ac.ir/article_31609.html L1 - https://jsciences.ut.ac.ir/article_31609_aa3cb65e8a5a782b89474faf041e84ee.pdf ER -