TY - JOUR ID - 88817 TI - Photo-Mediated Preparation of Silver Nanoparticles Dry Concentrate in an Organic Solvent System, Using Culture Supernatant of Klebsiella pneumonia: A Preliminary Study JO - Journal of Sciences, Islamic Republic of Iran JA - JSCIENCES LA - en SN - 1016-1104 AU - Mozafar, Maryam AU - Hajihosseini, Sajjad AU - Ajideh, Ramak AU - Yazdi, Mohammad Hossein AU - Minaeian, Sara AU - Shahverdi, Ahmad Reza AD - 1 Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Islamic Republic of Iran AD - 2 Biotechnology Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Islamic Republic of Iran AD - 3 Antimicrobial Resistance Research Center, Institute of Immunology and Infectious Diseases, Iran University of Medical Sciences, Tehran, Islamic Republic of Iran AD - 1 Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Islamic Republic of Iran and 2 Biotechnology Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Islamic Republic of Iran Y1 - 2022 PY - 2022 VL - 33 IS - 2 SP - 133 EP - 138 KW - Silver nanoparticles KW - Organic solvents KW - Biological synthesis KW - Klebsiella pneumonia KW - Dry concentrate DO - 10.22059/jsciences.2022.341769.1007723 N2 - Silver nanoparticles (AgNPs) are well-known nanomaterials that have been mainly used as antimicrobial agents Hundreds of chemical or biological methods have been reported and described for the preparation of AgNPs in water so far. Aqueous colloids of AgNPs normally have a limited capacity to safely change to the concentrated form using conventional methods such as different evaporation methods. Organic solvents which could be easily evaporated using conventional evaporation vacuum methods are good candidates for the preparation of highly concentrated AgNPs formulations or dried concentrates. In this study, we used a previously described biological method for preparing AgNPs in an acetone-water solvent mixture. The nanoparticles were synthesized using culture supernatant of Klebsiella pneumonia in the above organic solvent mixture supplemented with polyethylene glycol 6000 in a bright condition and subsequently dried by a conventional evaporation method. In the next step, dried residues were re-dispersed in water under ultra-sound treatment and characterized with different instrumentation methods. The results showed spherical AgNPs with a size range of ≤ 200 nm. This is the first report on the biological synthesis of AgNPs in an organic solvent mixture which could be easily converted to a dried form. This dried AgNPs concentrate is a good candidate for the preparation of very thick formulations of AgNPs such as solid or semi-solid pastes. UR - https://jsciences.ut.ac.ir/article_88817.html L1 - https://jsciences.ut.ac.ir/article_88817_4da6d2314e5c131e033b25669100c7e3.pdf ER -