University of Tehran Journal of Sciences, Islamic Republic of Iran 1016-1104 25 1 2014 03 01 TRIzol-based RNA Extraction: A Reliable Method for Gene Expression Studies 13 17 50483 EN M. Pahlevan Kakhki Department of Basic Sciences, Faculty of Medicine, Gonabad University of Medical Sciences, Gonabad, Islamic Republic of Iran Journal Article 2014 01 10 RNA extraction is a prerequisite technique for gene expression studies, analyzing the etiology and disease progression, treatment effects, as well as designing the diagnostic methods. Although many RNA extraction kits have been commercialized, but these kits are expensive and are not accessible in some countries. Many studies have shown that TRIzol is an applicable material for the RNA extraction from various biological samples. In this study, evaluation of the initial TRIzol volume and -20˚C temperature on the purity and solubility of RNA, which followed by measurement of <em>IL-1B</em> expression shows that TRIzol based RNA extraction is a “reliable” method for gene expression studies. https://jsciences.ut.ac.ir/article_50483_c0ec39be3a0080afa62cfcc304f8b6dd.pdf