University of Tehran Journal of Sciences, Islamic Republic of Iran 1016-1104 28 2 2017 04 01 Identification and Expression of Genes Involved in the Biosynthesis of Penicillin and Its Detection by HPLC in Penicillium chrysogenum 105 111 60746 EN SF. Miri 1Department of Plant protection, Faculty of Sciences, University of Sari Agricultural Sciences and Natural Resources, Sari, Islamic Republic of Iran M.A. Tajick 2Genetic and Agricultural Biotechnology Institute of Tabarestan (GABIT), University of Sari Agricultural Sciences and Natural Resources, Sari, Islamic Republic of Iran H. Rahimian 1Department of Plant protection, Faculty of Sciences, University of Sari Agricultural Sciences and Natural Resources, Sari, Islamic Republic of Iran G. Nematzadeh 2Genetic and Agricultural Biotechnology Institute of Tabarestan (GABIT), University of Sari Agricultural Sciences and Natural Resources, Sari, Islamic Republic of Iran Journal Article 2016 01 13 In this study, after identification of genes involved in biosynthesis of penicillin, we evaluated the expression of pcbAB and pcbC genes in <em>P.  Chrysogenum</em>.  A quantitative PCR (qPCR) approach was used to determine how these genes were expressed in different time courses. In addition, the produced penicillin content was measured using HPLC. qPCR analysis of mRNAs extracted from <em>P. chrysogenum</em> indicated that the expression  levels  of  pcbAB,  pcbC increased in seven days after inoculation compared  to  the  expressed levels with others time courses, but this difference was not significant statistically. The analysis of chromatograms from injected showed that the highest content of penicillin in media observed in 7 days after cultivation, except in <em>P. chrysogenum</em> PTCC 5033.  In addition, the results of comparative analysis among the tested strains showed that the penicillin content in 5, 7 and 11 days after cultivation was highest in  <em>P. chrysogenum</em> PTCC 5037, <em>P. chrysogenum</em> PTCC 5031 and <em>P. chrysogenum</em> PTCC 5033, respectively. However, the results showed an evident relationship between the expression levels of penicillin biosynthesis genes and yielded penicillin. secondary metabolites Penicillin gene expression HPLC https://jsciences.ut.ac.ir/article_60746_cbe931ae4f2d72807eed7c3bf04fdb4f.pdf