University of Tehran Journal of Sciences, Islamic Republic of Iran 1016-1104 31 3 2020 09 01 Evaluation of the Anticancer Effect of Xanthium Strumarium Root Extract on Human Epithelial Ovarian Cancer Cells Using 1H NMR-Based Metabolomics 205 212 76850 10.22059/jsciences.2020.291407.1007458 EN Roya Malekzadeh Metabolomics Lab. Biochemistry Dept. Pasteur INstitute of Iran, Dept. Of Biology, Payame Noor University, Tehran, Iran Mohammad Arjmand Metabolomics Lab. Biochemistry Dpt. Pasteur Institute of Iran Reza Haji Hosseini Dept. of Biology, Payame Noor University, Tehran, Iran Atusa Vaziri Dept. of Biology, Payame Noor University, Tehran, Iran Zahra Zamani Metabolomics Lab. Biochemistry Dept. Pasteur Institute of Iran, Tehran, Iran Journal Article 2019 11 09 Epithelial Ovarian cancer is the leading cause of cancer mortality among women all over the world. As chemotherapeutics has many side effects, researchers have focused on the potential use of medicinal plants as natural antitumor agents. <em>Xanthium strumarium</em> studied in this work as an herbal anticancer agent. This study aimed to evaluate the antitumor effect and metabolic alterations caused by the root extract of <em>X. strumarium</em> on human ovarian cancer cell line (A2780cp), using NMR-based metabolomics approaches. Cells were cultured and treated with different concentrations of the ethanolic plant extract. Antitumor activity determined by MTT assay and cell metabolites extracted for NMR spectroscopy. ¹H NMR spectroscopy was applied, and outliers were analyzed using multivariate statistical analysis techniques. The extract exhibited antitumor activity against ovarian cancer cells with an IC₅₀of 6 μg/ml after 48 hours of treatment_. The most affected metabolic pathways in the experimental groups were limited to tyrosine metabolism, nucleotide metabolism, fatty acid biosynthesis, and glycerolipid metabolism. Our data showed that the ethanolic root extract of <em>X. strumarium</em> has antitumor activity on the ovarian cancer cells and can affect vital metabolic pathways. However, further studies required to validate this activity. https://jsciences.ut.ac.ir/article_76850_b45f5c9dbf64f0265f5407e91ddf92a9.pdf