University of Tehran Journal of Sciences, Islamic Republic of Iran 1016-1104 24 4 2013 12 01 Biodecaffeination by Pseudomonas pseudoalcaligenes TPS8, an Isolated Strain from Tea Plantation Soil 305 312 36448 EN Morahem Ashengroph Department of Biology, Faculty of Sciences, University of Kurdistan Sajad Ababaf University of Kurdistan Journal Article 2013 09 28 Development of an environmental friendly and cost-effective process for microbial degradation of caffeine to non-toxic compounds are promising to solve the problems of physiochemical extraction of caffeine in the treatment the caffeine containing agro-industrial effluents. Thirteen bacterial strains, isolated from tea plantation soils in the north region of Iran, were screened to show their abilities in using caffeine as the sole source of carbon and nitrogen. The intrinsic tolerance of the isolated strains to the caffeine substrate was measured in a defined and complex medium by using the agar dilution method. Based on the tolerance efficiency, isolate TPS8 which showed maximum tolerance to caffeine was selected and identified as Pseudomonas pseudoalcaligenes strain TPS8 (GenBank accession number KF414528) according to the cultural and physiochemical characteristics and also 16S rDNA gene sequencing. Growing cells of P. pseudoalcaligenes TPS8 were used for the biodecaffeination experiments. The maximum removal of caffeine (80.2%) was reached after a 72 h incubation using 2.5 g/l of caffeine substrate without further optimization. Our results show that growing cells of P. pseudoalcaligenease TPS8 can thus be efficiently used as a simple and cheap process for preparative decaffeination from agro-industrial effluents. The present survey is the first report on biodecaffeination using Pseudomonas pseudoalcaligenes.

https://jsciences.ut.ac.ir/article_36448_2d28fa88660dc11bb28fc850b65b9ac1.pdf

University of Tehran Journal of Sciences, Islamic Republic of Iran 1016-1104 24 4 2013 12 01 The Different Antibacterial Impact of Silver Nanoparticles Against Legionella pneumophila Compared to Other Microorganisms 313 319 36449 EN M.R. Pourmand Department of Pathobiology, School of Public Health, and Biotechnology Research Center, Tehran University of Medical Sciences, Tehran, Islamic Republic of Iran K. Shahidi Department of Pharmaceutical Biotechnology and Biotechnology Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Islamic Republic of Iran P. Nazari Department of Pharmaceutical Biotechnology and Biotechnology Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Islamic Republic of Iran Seyed M. Moosavian Department of Microbiology, School of Medicine and Tropical Research Center, Ahwaz Jundishapour University of Medical Sciences, Ahwaz, Islamic Republic of Iran N. Samadi Department of Drug and Food Control and Quality Assurance Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Islamic Republic of Iran Gh. Pourmand Urology Research Center, Sina Hospital, Tehran University of Medical Sciences, Tehran, Islamic Republic of Iran A. R. Shahverdi Department of Pharmaceutical Biotechnology and Biotechnology Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Islamic Republic of Iran Journal Article 2013 01 21 Legionella pneumophila is the pathogen responsible for severe pneumonia known as Legionnaires’ disease. Legionella can live under varied stress conditions, especially in cold environments, and is common in many artificial environments. In this study, the antimicrobial activity of biogenic silver nanoparticles, prepared using the culture supernatant of Klebsiella pneumoniae, was evaluated against different important microorganisms, especially L. pneumophila. Compared with all bacterial test strains, L. pneumophila showed lower susceptibility against silver nanoparticles (MIC 30 µg/mL). The MICs of silver nanoparticles were 10 µg/ml for Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Serratia marcescens, and Salmonella typhi. In addition, the MIC values of the silver nanoparticles for the fungal teststrain(Aspergillus niger and Candida albicans) were 20 and 30 µg/ml, respectively. The time-kill course antibacterial assay of silver nanoparticles at its MIC concentration (30 µg/mL) was performed against L. pneumophila at cold (4 °C) and ambient (25 °C) temperatures. The results showed that a higher mortality rate was observed at 4 °C. In contrast, at 25 °C,a considerable tolerance was observed for L. pneumophila against 30 µg/ml of silver nanoparticles during the 24-h exposure time. Reserpine as a natural efflux inhibitor (10 µg/mL) was used in this study to reverse the resistance of L. pneumophila against silver nanoparticles at 25 °C. Higher anti-legionella activity was detected in the presence of reserpine at the mentioned temperature that indicated that efflux pumps may have an important role in the resistance of L. pneumophila against silver nanoparticles at 25 °C.

https://jsciences.ut.ac.ir/article_36449_7e67de4c7bdc4f9f173be3e29cb8c95e.pdf

University of Tehran Journal of Sciences, Islamic Republic of Iran 1016-1104 24 4 2013 12 01 Karyotype of Hairless Guinea pig 321 324 36450 EN Fatemeh - Todehdehghani Reproductive biology Mohammah Hassan Motedayen immunology Shohreh Teimorzadeh Biology Journal Article 2013 10 04 Chromosomal patterns of experimental animals are useful tools for cytogenetics research and animal breeding. Chromosome investigations of the hairless guinea pig are rare, therefore, karyotype of hairless guinea pigs (twelve male and female) was studied using metaphase spreads of bone marrows and G banding techniques. The chromosomes diploid number was 2n= 64 and polymorphism of three type chromosomal pairs were observed in the genus Cavia of Iranian hairless guinea pigs. A karyotype of 24 biarmed pairs and seven pairs of acrocentric chromosomes, 2 of sex chromosomes were also seen. The findings describe the karyology of the hairless guinea pig that is produced by Razi vaccine and serum research institute of Iran that improves our knowledge about laboratory hairless guinea pig and provides basic data for further use of the animal.

https://jsciences.ut.ac.ir/article_36450_e772c80520cf6e7f205e226da42115b3.pdf

University of Tehran Journal of Sciences, Islamic Republic of Iran 1016-1104 24 4 2013 12 01 Formation and stability of organic layers around inorganic particles in aqueous media: an introduction to origin of life 325 333 36451 EN Farnaz Jahanbin Ardabil Branch, Islamic Azad University Mohammad Khodadadi-Moghaddam Ardabil Branch, Islamic Azad University Shahab Bohlooli Ardabil University of Medical Sciences Journal Article 2013 11 26 Formation of non bonded organic layer around inorganic particles like iron sulfide and zeolites, was hypothesized as starting point for one of the possible mechanisms of the origin of life on earth. In this study, the general circumstances needed for creation of such organic layer was investigated. An aliphatic hydrocarbon (hexane), an aromatic hydrocarbon (toluene), an alcohol (n-octanol) and an amino acid (phenylalanine) were used for simulation of the primordial soup. FeS, montmorillonite and clinoptilolite were used as insoluble inorganic particles. Non-bonded organic layer can be formed in both fresh and saline waters. Formation of organic layer is a pH sensitive process and the best pH for formation of this layer is around 6 (similar to the pH of primordial oceans of the earth). Low concentration of water in organic layer causes the less degradation rate of the polymers and various organic molecules and thus increases their stability.

https://jsciences.ut.ac.ir/article_36451_f76ebceb212748f03d8487120fdc667f.pdf

University of Tehran Journal of Sciences, Islamic Republic of Iran 1016-1104 24 4 2013 12 01 Composition of volatile compounds of extract of Ammi majus from Iran by GC-MS 335 338 36453 EN Sh. Nayebi Plant Breeding and Biotechnology Department, Faculty of Agriculture, University of Tarbiat Modares, Tehran, Islamic Republic of Iran T. Kakeshpour Plant Breeding and Biotechnology Department, Faculty of Agriculture, University of Tarbiat Modares, Tehran, Islamic Republic of Iran A. Hasanvand company of Zagros Daru, Lorestan, Aleshtar, Islamic Republic of Iran M. Nadri company of Zagros Daru, Lorestan, Aleshtar, Islamic Republic of Iran S. Rashidi Monfared company of Zagros Daru, Lorestan, Aleshtar, Islamic Republic of Iran Journal Article 2013 06 18 Abderaz The composition of the essential oil obtained from the seeds of Ammi majus L. which collected from province of Khozestan in the southwest area of Iran.The oils was analyzed by gas chromatography mass spectrometry (GC-MS). Twenty - eight components have been identified in the extract of A. majus L. with Toluene (3.766%), Thymol (12.811%), Carvacrol (37.811%) as major components, respectively. The seeds extract was rich in monoterpenes. The various functions of terpenoide compounds have been widely studied. They have diverse activity including antibacterial, antifungal, antinociceptive, anti-inﬂammatory relieving digestive and anticancer.

https://jsciences.ut.ac.ir/article_36453_f3ea77c9db071620d377b5431ea65e3e.pdf

University of Tehran Journal of Sciences, Islamic Republic of Iran 1016-1104 24 4 2013 12 01 Heavy metal concentration of soils affected by Zn-smelter activities in the Qeshm Island, Iran 339 346 36454 EN F. Moore Department of Earth Sciences, Faculty of Sciences, Shiraz University, Shiraz, Islamic Republic of Iran S. Kargar Department of Earth Sciences, Faculty of Sciences, Shiraz University, Shiraz, Islamic Republic of Iran F. Rastmanesh Department of Earth Sciences, Faculty of Sciences, Shahid Chamran University, Ahvaz, Islamic Republic of Iran Journal Article 2013 06 08 In this study, concentrations of heavy metals in soils around a Zn-smelter in Qeshm island, Iran, are investigated. Calculated geoaccumulation index (Igeo), contamination factor (Cf), and contamination degree (Cdeg) values indicate surface soil contamination by toxic metals (As, Cd, Pb, and Zn). The results also indicate that most contaminated areas are located in the vicinity of the smelter and waste pile. However, concentrations of soil metals decrease with increasing distance from the pollution sources. Results from a potential ecological risk assessment indicate high risk in areas around the smelter. Statistical analysis also confirms the role of the Zn-smelter in soil contamination in the study area.

https://jsciences.ut.ac.ir/article_36454_701f786e616388db90ecf84f45fc2f13.pdf

University of Tehran Journal of Sciences, Islamic Republic of Iran 1016-1104 24 4 2013 12 01 Modified F-Expansion Method Applied to Coupled System of Equation 347 354 36455 EN A. Aasaraai Department of Applied Mathematics, Faculty of Mathematical Sciences, University of Guilan, Rash, Islamic Republic of Iran M.B. Mehrlatifan Department of Applied Mathematics, Faculty of Mathematical Sciences, University of Guilan, Rash, Islamic Republic of Iran S. Khaleghizadeh Modified F-Expansion Method Applied to Coupled System of Equation Journal Article 2013 02 07 A modified F-expansion method to find the exact traveling wave solutions of two-component nonlinear partial differential equations (NLPDEs) is discussed. We use this method to construct many new solutions to the nonlinear Whitham-Broer-Kaup system (1+1)-dimensional. The solutions obtained include Jacobi elliptic periodic wave solutions which exactly degenerate to the soliton solutions, triangular periodic wave solutions, exponential solutions and rational solutions under certain limitet conditions. In addition, some figures of partial solutions are provided for direct-viewing analysis. The method can also be extended to other types of nonlinear evolution equations in mathematical physics.

https://jsciences.ut.ac.ir/article_36455_7777da9c4ae4d5edbbfe88796800cdf4.pdf

University of Tehran Journal of Sciences, Islamic Republic of Iran 1016-1104 24 4 2013 12 01 RAWSN: A Routing Algorithm Based on Auxiliary Nodes to Reduce Energy Consumption in Wireless Sensor Networks 355 359 36456 EN A.A. Baradaran Department of Computer sciences ,Factualy of sciences, Islamic Azad university,Natanz, Islamic Republic of Iran Journal Article 2013 06 08 In this paper, an algorithm , based on genetics and auxiliary nodes, to reduce energy consumption in wireless sensor networks has been presented. In this research, by considering some parameters as energy and distance, a target function has been created, which is more optimum comparing to other methods. In this research cluster head is selected by genetic algorithm. In RAWSN algorithm a new technique of replacing auxiliary nodes when some CHs ’energies come to an end, is presented. The results of simulation show that the number of alive nodes at the end of each round increases comparing to other methods and this has led to prolongation of network lifetime.

https://jsciences.ut.ac.ir/article_36456_e0440b350e347a5fb95e380c8d3df809.pdf

University of Tehran Journal of Sciences, Islamic Republic of Iran 1016-1104 24 4 2013 12 01 Some properties of n-capable and n-perfect groups 361 364 36457 EN M. R. Rismanchian Department of Pure Mathematics, Faculty of Mathematical Sciences, Shahrekord University, Shahrekord, Islamic Republic of Iran. Journal Article 2013 07 07 In this article we introduce the notion of n-capable groups. It is shown that every group G admits a uniquely determined subgroup (〖Z^n)〗^* (G) which is a characteristic subgroup and lies in the n-centre subgroup of the group G. This is the smallest subgroup of G whose factor group is n-capable. Moreover, some properties of n-central extension will be studied.

https://jsciences.ut.ac.ir/article_36457_52c4a08f484812fbb2c22163f5342e7b.pdf