University of TehranJournal of Sciences, Islamic Republic of Iran1016-110427420161101Screening of Potentially Probiotic Lactobacillus Possessing Surface Layer Protein from Iranian Traditional Dairy Products30531259336ENE.Mobarak QamsariDepartment of Microbiology, Faculty of Biology, Alzahra University, Tehran, Islamic Republic of IranR.Kasra KermanshahiDepartment of Microbiology, Faculty of Biology, Alzahra University, Tehran, Islamic Republic of IranM.ErfanDepartment of Pharmaceutics, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Islamic Republic of IranP.GhadamDepartment of Biotechnology, Faculty of Biology, Alzahra University, Tehran, Islamic Republic of IranJournal Article20150619One of the most commonly observed surface structures on the prokaryotic cell envelopes is monomolecular crystalline array of proteinaceous subunits termed Surface Layers or S-layers. Due their self assembly ability and the highly ordered, regular structure down to the nanometer scale, S-layers were demonstrated to possess a great potential for nanobiotechnological applications. Some probiotic bacteria, such as <em>Lactobacillus</em> spp. have been found to possess S-layers. In this perspective, the objectives of this study were isolation and identification of <em>Lactobacillus</em> species from traditional dairy products carrying surface (S) layer protein and investigation of important prerequisite of probiotic interest, such as the capability to survive at low pH and in presence of bile salts. The protein profile of intact <em>Lactobacillus </em>isolates was analyzed in SDS-PAGE. The protein bands of 45–60 kDa were present in two of the 25 isolates, suggesting the presence of S-layer proteins in these strains. The above mentioned proteins were recovered in the guanidine hydrochloride extracts. After dialysis, the extracted proteins showed a significant band of apparent molecular mass of 50–60 kDa in SDS-PAGE. The strains possessing S-layers protein was identified both biochemically and by 16S rRNA sequencing. The 16S rRNA gene sequence analysis revealed that two isolates exhibited maximum similarity with the 16S rRNA sequence of <em>Lactobacillus brevis</em> spp. These two isolates were resistant to acid and bile in which their viability in acidic condition were between 51.6% and 77.8% and bile resistance of two isolates was approximately 99% after 24 h.https://jsciences.ut.ac.ir/article_59336_71b67bf75e223a77f1a12baf4062dd2b.pdfUniversity of TehranJournal of Sciences, Islamic Republic of Iran1016-110427420161101Transformation And Light Inducible Expression of cry1Ab Gene in Oilseed Rape (Brassica napus L.)31331959337ENH.RahnamaAgricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research Education and Extension Organizatior (AREEO), Karaj, Islamic Republic of IranM.SheykhhasanSari Agricultural Sciences and Natural Resources University (SANRU), Islamic Republic of IranJournal Article20150906Rapeseed (<em>Brassica napus</em> L.) is the third most important oil crop in global productions. One of the major limiting factors for oilseed rape production is lepidopteran pests of the <em>Brassicaceae</em> family. Transgenic plants expressing <em>Bacillus thuringiensis</em> (Bt) genes are powerful tools in the integrated pest management of crop plants. In the present study, we used a synthetic Bt insecticidal crystal protein gene (<em>cry1Ab</em>) under the control of the <em>phosphoenolpyruvate carboxylase</em> (PEPC) promoter for genetic transformation of <em>B. napus </em>L. (var. SLM046) using <em>Agrobacterium tumefaciens</em>-mediated transformation. PEPC-cry1Ab-nos cassette cloned in a binary vector pCAMBIA3300 containing <em>bar</em> gene as a selection marker. The new vector (pCAMBIAPEPCry) introduced to AGL01 strain of <em>A. tumefaciens</em>, which used in transformation of hypocotyl explants of <em>B. napus</em>. Putative transgenic rapeseed plants were regenerated in selection media containing phosphinothricin (PPT) as selection agent. Polymerase Chain Reaction (PCR) confirmed the integration of <em>cry1Ab</em> and <em>bar</em> genes at putative transgenic plant genome. Furthermore, transcription (mRNA production) and protein expression of <em>cry1Ab</em> gene was confirmed using RT-PCR and immune-strip methods, respectively. Transgenic <em>B. napus</em> plants expressed Cry1Ab protein in the shoots and not in the roots. We concluded that C4 maize PEPC promoter can induce the expression of Cry1Ab recombinant protein only in light treated (green) tissues in rapeseed plants. It recomended as a light inducible promoter for targeted expression of transgene in the rapeseed plants. https://jsciences.ut.ac.ir/article_59337_51e82935e8089944e4633f85546e85d3.pdfUniversity of TehranJournal of Sciences, Islamic Republic of Iran1016-110427420161101Potentiometric Determination of Acidity Constants of Some Synthesized Organic Compounds in Organic-Water Media32132859338ENSh.BalujaPhysical Chemistry Laboratory, Department of Chemistry, Saurashtra University, Rajkot-360005 (Gujarat), IndiaP.RamavatPhysical Chemistry Laboratory, Department of Chemistry, Saurashtra University, Rajkot-360005 (Gujarat), IndiaK.NandhaPhysical Chemistry Laboratory, Department of Chemistry, Saurashtra University, Rajkot-360005 (Gujarat), IndiaJournal Article20151213<span>The acidity constants of some synthesized protonated pyrazolo quinazoline compounds were determined potentiometrically at ionic strength of 0.1 M in DMF: water (60:40 v/v) system at different temperatures (298.15 K to 318.15 K). The pKa values have been found to increase with increasing electron-donating nature of substitutions. Some thermodynamics parameters such as enthalpy (ΔH°), Gibb’s free energy (ΔG°) and entropy (ΔS°) of reactions have also been evaluated at different temperatures for these systems. The thermodynamic parameters indicate that dissociation process is not spontaneous and exothermic. </span>https://jsciences.ut.ac.ir/article_59338_959a946104469c738e1f27fb8180d561.pdfUniversity of TehranJournal of Sciences, Islamic Republic of Iran1016-110427420161101Possible Basement Transverse Faults in the Western Alborz, Northern Iran32934259339ENM.Ehteshami-MoinabadiDepartment of Geology, Faculty of Earth Sciences, Shahid Beheshti University, Tehran, Islamic Republic of IranJournal Article20160109<span>Transverse basement (TB) faults are important structures in the mountain belts or sedimentary basins influencing various aspects of them. The origin of these faults is diverse, but their effect on the shape and configuration of continental margins is characteristics. The Western Alborz range that borders the South Caspian basin to southwest is a complex range with principal faults and known earthquakes such as 1990 Rudbar (Mw 7.3). However, TB faults are less known in this range. This paper attempts to compile available data from aeromagnetic survey, local geology maps, tectonic maps, remote sensing and earthquake data to introduce several important TB faults or lineament in the Western Alborz. Qezel Owzan-West Talesh, Lahijan-Sepidrud, Takestan-Polrud and Valian-Hezar are large TB faults or lineaments across the Western Alborz. Some of introduced TB faults and lineaments are possibly continuing within the South Caspian basin. The TB faults introduced or assessed in this paper are more or less correlating with irregularities of the northern margin of the Alborz Range. This correlation is clearer for the Lahijan-Sepidrud TB fault, F-1lineament and to a lesser degree for Amlash, Polrud-Takestan and Valian-Hezar lineaments. Detail geometry, origin, effect on sedimentation along the continental margin and their relationship with huge regional magmatism in the Cretaceous and Eocene in the Western Alborz suggested subjects for further investigations on these faults and lineament.</span>https://jsciences.ut.ac.ir/article_59339_fd88742ff66fe022300b3cacfde7e7bd.pdfUniversity of TehranJournal of Sciences, Islamic Republic of Iran1016-110427420161101Study of Zircon Crystals: An Implication to Determine of Source and Temperature of Crystallization in Turkeh Dareh Pluton, NW Iran34335259340ENM.JamshidibadrDepartment of Geology, Faculty of Sciences, University of Payam Noor,Tehran, Islamic Republic of IranJournal Article20160523 Turkeh Dareh pluton is outcrop in NW of Iran and it is one of the intrusions in Sanandaj-Sirjan zone. Shaped crystals of this pluton distinct oscillatory zoning narrow and close together zoning in zircons indicate parental magma richness of zirconium. Factors affecting the shape of the zircon crystals are the composition, possibly the temperature of the crystallization, water content in magma and origin of the magma, therefore, were studied morphology of zircon crystals of Turkeh Dareh pluton. Morphologically, most of Turkeh Dareh zircons are located in range of S13 and S14 and in lesser extent in the range of S18, S19 and S20. The minimum zircons crystallization temperature was calculated 750 to 840 Cº based on the morphology of zircon, zircon saturation and the whole rock geochemistry. The origin of intrusion magma of Turkeh Dareh is I-type and calc-alkaline that these results are consistent with results of field observations, mineralogy and geochemistry of this pluton.https://jsciences.ut.ac.ir/article_59340_7e6568c7b3462710eb57d9955a12a6b1.pdfUniversity of TehranJournal of Sciences, Islamic Republic of Iran1016-110427420161101A Characterization of the Suzuki Groups by Order and the Largest Elements Order35335559341ENB.EbrahimzadehDepartment of Mathematics, Faculty of Basic Sciences, Science and Research Branch, Islamic Azad University, Tehran, Islamic Republic of IranA.IranmaneshDepartment of Mathematics, Faculty of Mathematical Sciences, Tarbiat Modares University, Tehran, Islamic Republic of IranA.TehranianDepartment of Mathematics, Faculty of Basic Sciences, Science and Research Branch, Islamic Azad University, Tehran, Islamic Republic of IranH.Parvizi MosaedAlvand Institute of Higher Education, Hamedan, Islamic Republic of IranJournal Article20160514One of the important problems in group theory is characterization of a group by a given property, that is, to prove there exist only one group with a given property. Let be a finite group. We denote by the largest order of elements of . In this paper, we prove that some Suzuki groups are characterizable by order and the largest order of elements. In fact, we prove that if is a group with and where or is a prime number, then .https://jsciences.ut.ac.ir/article_59341_6da908067023f8e9313fc102eaf309a4.pdfUniversity of TehranJournal of Sciences, Islamic Republic of Iran1016-110427420161101Admissibility of Linear Predictors of Finite Population Parameters under Reflected Normal Loss Function35536659342ENR.JafaraghaieDepartment of Statistics, Faculty of Mathematical Sciences and Computer, Allameh Tabataba’i University, Tehran, Islamic Republic of IranN.NematollahiDepartment of Statistics, Faculty of Mathematical Sciences and Computer, Allameh Tabataba’i University, Tehran, Islamic Republic of IranJournal Article20150802One of the most important prediction problems in finite population is the prediction of a linear function of characteristic values of a finite population. In this paper the admissibility of linear predictors of an arbitrary linear function of characteristic values in a finite population under reflected normal loss function is considered. Under the super-population model, we obtain the conditions for which the linear predictors are admissible. Also, the risk of some admissible and inadmissible predictors are compared by a simulation study.https://jsciences.ut.ac.ir/article_59342_eeb56d29286995142e72c0e45310ca7c.pdfUniversity of TehranJournal of Sciences, Islamic Republic of Iran1016-110427420161101Author Index37738559343ENJournal Article20161112https://jsciences.ut.ac.ir/article_59343_84e8c75f470ff91f39b02c1976a85091.pdfUniversity of TehranJournal of Sciences, Islamic Republic of Iran1016-110427420161101Keywords Index38639659344ENJournal Article20161112https://jsciences.ut.ac.ir/article_59344_c438c0e15652b4588246d46e44b24c83.pdf