Genotyping for five short tandem repeat (STR) loci HUMvWA, HUMFES, HUMTPO, HUMTH01 and D3S1359 was done in 220 unrelated individuals from the population of Isfahan province of IR Iran. The loci were genotyped using the polymerase chain reaction (PCR) followed by polyacrylamide gel electrophoresis (PAGE) and silver staining. The data demonstrated that the STR markers were all found informative in the population examined. The markers were also found to have a relatively high degree of heterozygosity. Forensic and paternity indices including power of discrimination (PD) and exclusion (PE) as well as polymorphism information content (PIC) and typical paternity index were determined for the examined STR alleles. Together, the examined STR loci in this study could be considered for paternity testing and individual identification in Iranian population. Moreover, the data could be used in construction of the first Iranian STR genetic database.