Introducing a New and Simple Protocol for Capillary Electrophoresis of Cell Free Fetal Double Stranded DNA

Document Type: Original Paper

Authors

Department of Medical Genetics, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Islamic Republic of Iran

Abstract

Isolation of cell free fetal DNA (cffDNA) from maternal serum usually leads to very low concentrations of DNA impeding further resolving through conventional methods of electrophoresis. Although several protocols have been described for capillary electrophoresis (CE) of double stranded DNA, they usually need using special polymers or coated capillaries which degrade over time. Herein, we proposed a new and very simple protocol using uncoated fused silica capillary for CE of cffDNA purified from serum of pregnant women. The proposed protocol was able to determine the presence of small fetal DNA fragments and it also confirmed the optimization of sonication performed on purified cffDNA samples. 

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