Gene Expression Profile of Adherent Cells Derived From Human Peripheral Blood: Evidence of Mesenchymal Stem Cells

Document Type : Final File

Authors
Z. Fazeli 1
M. Rajabibazl 2
S. Salami 2
N. Vazifeh Shiran 3
SMH. Ghaderian 4
MD. Omrani 4
1 1 Department of Medical Genetics, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Islamic Republic of Iran
2 2 Department of Clinical Biochemistry, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Islamic Republic of Iran
3 3 Department of Hematology, School of Allied Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Islamic Republic of Iran
4 4 Urogenital stem cell research center, Shahid Beheshti University of medical sciences, Tehran, Islamic Republic of Iran
Abstract
Mesenchymal stem cells (MSCs) provide a novel option in cellular therapy and tissue engineering. Recent studies indicated that it is possible to obtain MSCs from peripheral blood by attachment ability to plastic surface. To evaluate adherent cells derived from peripheral blood, their expression profile and surface markers were investigated. The results of RT-PCR indicated that these cells expressed MECP2, SLITRK2, SLITRK4 as well as the neuronal markers Nestin, β III tubulin, NFM, MAP2 and NSE. In the present study, a panel of surface markers were analysed by flow cytometry. These cells display a fibroblast like morphology as well as known sources of MSCs. The results obtained from immune-staining represented that these cells were negative for CD14, CD45, CD15 and CD24, whereas they were positive for CD29, CD44, CD105, CD73, CD166 and CD184. Our data indicated that the peripheral blood could be considered as a source of MSCs with high yield.
Keywords
Mesenchymal stem cells
Neuronal markers
Expression profile
Immune-phenotyping
Journal of Sciences, Islamic Republic of Iran
Volume 27, Issue 2 - Serial Number 2
Spring 2016
Pages 105-112