Document Type : Original Paper
1 Department of Clinical Sciences, Faculty of Specialized Veterinary Sciences, Science and Research Branch, Islamic Azad University, Tehran, Islamic Republic of Iran
2 Department of Basic Sciences, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Islamic Republic of Iran
3 Department of Pathology, Faculty of Specialized Veterinary Sciences, Science and Research Branch, Islamic Azad University, Tehran, Islamic Republic of Iran
4 Department of Microbiology, Faculty of Basic Science, Saveh Branch, Islamic Azad University, Markazi, Islamic Republic of Iran
Antibiotic resistance is among the most concerning issues worldwide. Currently, the use of natural alternatives with improved therapeutic effects and fewer complications than common therapies is considered a novel therapeutic approach to care urinary tract infections (UTI). In this research, we evaluated the prospective activity of Oliveria decumbens vent essential oil (ODEO) in the treatment of Escherichia coli-induced cystitis. The antibacterial properties of ODEO were investigated using standard microdilution assays against E. coli. To induce cystitis, 1.5 ×108 CFU/ml of E. coli (ATCC 700928) was injected into the bladder of Wistar rats, and then they were prescribed ODEO and gentamicin. The histopathological parameters of the bladders were tested at the end of the study. The MIC and MBC of the ODEO against E.coli were 0.54 μl/ml and 1.024 μl/ml respectively. In the infected group with no treatment, the infiltration of inflammatory cells and the thickness of bladder tissue were increased notably, however in the groups treated with ODEO, especially at higher doses, the parameters were significantly decreased (p<0.01). The ODEO efficacy was comparable to gentamicin in the reduction of the bacterial count. In addition, after administration of the ODEO, inflammation, fibrosis, and thickness of epithelium also decreased in a dose-dependent manner (p<0.01). In conclusion, treatment with ODEO which added as drinking supplement or injected subcutaneously resulted to the renal clearance of pathogens in study rats.
- Lüthje P, Brauner A. Novel strategies in the prevention and treatment of urinary tract infections. Pathogens. 2016 Jan 27;5(1):13.
- Mishra MP, Rath S, Swain SS, Ghosh G, Das D, Padhy RN. In vitro antibacterial activity of crude extracts of 9 selected medicinal plants against UTI causing MDR bacteria. J. King Saud Univ. Sci. 2017 Jan 1;29(1):84-95.
- Ramos NL, Dzung DT, Stopsack K, Jankó V, Pourshafie MR, Katouli M, Brauner A. Characterisation of uropathogenic Escherichia coli from children with urinary tract infection in different countries. Eur. J. Clin. Microbiol. 2011 Dec;30(12):1587-93.
- Behbahani BA, Yazdi FT, Vasiee A, Mortazavi SA. Oliveria decumbens essential oil: Chemical compositions and antimicrobial activity against the growth of some clinical and standard strains causing infection. Microb. Pathog. 2018;114:449-52.
- Miguel MG. Antioxidant and anti-inflammatory activities of essential oils: a short review. Molecules. 2010 Dec 15;15(12):9252-87.
- Shaheen G, Akram M, Jabeen F, Ali Shah SM, Munir N, Daniyal M, Riaz M, Tahir IM, Ghauri AO, Sultana S, Zainab R. Therapeutic potential of medicinal plants for the management of urinary tract infection: A systematic review. Clin. Exp. Pharmacol. Physiol. 2019 Jul;46(7):613-24.
- Jamali T, Kavoosi G, Jamali Y, Mortezazadeh S, Ardestani SK. In-vitro, in-vivo, and in-silico assessment of radical scavenging and cytotoxic activities of Oliveria decumbens essential oil and its main components. Sci. Rep. 2021 Jul 12;11(1):1-9.
- Zahabi N, Golmakani MT, Fazaeli M, Ghiasi F, Khalesi M. Electrospinning of glutelin-hordein incorporated with Oliveria decumbens essential oil: Characterization of nanofibers. Colloids Surf. B. 2021 Dec 1;208:112058.
- Jamali T, Kavoosi G, Ardestani SK. In-vitro and in-vivo anti-breast cancer activity of OEO (Oliveria decumbens vent essential oil) through promoting the apoptosis and immunomodulatory effects. J. Ethnopharmacol. 2020;248:112313.
- Karami A, Kavoosi G, Maggi F. The emulsion made with essential oil and aromatic water from Oliveria decumbens protects murine macrophages from LPS-induced oxidation and exerts relevant radical scavenging activities. Biocatal. Agric. Biotechnol. 2019;17:538-44.
- Eftekhari M, Ardekani MRS, Amin M, Attar F, Akbarzadeh T, Safavi M, et al. Oliveria decumbens, a bioactive essential oil: Chemical composition and biological activities. Iran. J. Pharm. Sci. 2019;18(1):412.
- Khoshbakht T, Karami A, Tahmasebi A, Maggi F. The variability of thymol and carvacrol contents reveals the level of antibacterial activity of the Essential Oils from different accessions of Oliveria decumbens. Antibiotics. 2020;9(7):409.
- Tuken M, Temiz MZ, Yuruk E, Kaptanagasi AO, Basak K, Narter F, Muslumanoglu AY, Sarica K. The role of an herbal agent in treatment for Escherichia coli induced bacterial cyctitis in rats. Arch. Ital. Urol. 2017 Jun 30;89(2):134-8.
- Smaldone MC, Vodovotz Y, Tyagi V, Barclay D, Philips BJ, Yoshimura N, Chancellor MB, Tyagi P. Multiplex analysis of urinary cytokine levels in rat model of cyclophosphamide-induced cystitis. Urology. 2009 Feb 1;73(2):421-6.
- Ceprnja M, Oros D, Melvan E, Svetlicic E, Skrlin J, Barisic K, et al. Modeling of urinary microbiota associated with cystitis. Front. Cell. Infect. Microbiol. 2021;11:643638.
- Hayami H, Takahashi S, Ishikawa K, Yasuda M, Yamamoto S, Wada K, et al. Second nationwide surveillance of bacterial pathogens in patients with acute uncomplicated cystitis conducted by Japanese Surveillance Committee from 2015 to 2016: antimicrobial susceptibility of Escherichia coli, Klebsiella pneumoniae, and Staphylococcus saprophyticus. J. Infect. Chemother. 2019;25(6):413-22.
- Del Popolo G, Nelli F. Recurrent bacterial symptomatic cystitis: A pilot study on a new natural option for treatment. Arch. Ital. Urol. 2018;90(2):101-3.
- Stothers L. A randomized trial to evaluate effectiveness and cost effectiveness of naturopathic cranberry products as prophylaxis against urinary tract infection in women. Can J Urol. 2002 Jun 1;9:1558-62.
- Havranova J, Krinock M, Widawski M, Sluder R, Kumar A, Hippen J, Goel H. Cranberry Extract for Preventing Recurrent Urinary Tract Infections: An Outcome-Specific Meta-Analysis of Prospective Trials. J. women's health dev. 2020;3(3):222-42.
- Sappal S, Goetz LL, Vince R, Klausner AP. Randomized trial of concentrated proanthocyanidins (PAC) for acute reduction of bacteriuria in male veterans with spinal cord injury utilizing clean intermittent catheterization. Spinal cord ser. cases. 2018 Jun 28;4(1):1-6.
- Rafiee S, Ramezanian A, Mostowfizadeh-Ghalamfarsa R, Niakousari M, Saharkhiz MJ, Yahia E. Nano-emulsion of denak (Oliveria decumbens Vent.) essential oil: ultrasonic synthesis and antifungal activity against Penicillium digitatum. J. Food Meas. Charact. 2022 Feb;16(1):324-31.
- Nabavi SM, Marchese A, Izadi M, Curti V, Daglia M, Nabavi SF. Plants belonging to the genus Thymus as antibacterial agents: From farm to pharmacy. Food chem. 2015 Apr 15;173:339-47.
- Ballester-Costa C, Sendra E, Fernández-López J, Pérez-Álvarez JA, Viuda-Martos M. Chemical composition and in vitro antibacterial properties of essential oils of four Thymus species from organic growth. Ind Crops Prod. 2013 Oct 1;50:304-11.
- Hirota R, Roger NN, Nakamura H, Song HS, Sawamura M, Suganuma N. Anti‐inflammatory effects of limonene from yuzu (Citrus junos Tanaka) essential oil on eosinophils. J Food Sci. 2010;75(3):H87-H92.
- Mahboubi M, Heidarytabar R, Mahdizadeh E, Hosseini H. Antimicrobial activity and chemical composition of Thymus species and Zataria multiflora essential oils. Agr Nat Res. 2017 Oct 1;51(5):395-401.