In vitro antimicrobial estimation of some methyl 2-methyl-6-oxo-4-phenyl-1,4,5,6-tetrahydropyridine-3- carboxylate derivatives

Sadeghi-aliabadi, Hojjat; Sadeghipour, Marzieh; Abbasi, Maryam; Mahernia, Shabnam; Shafiee, Fatemeh

doi:10.22059/jsciences.2025.391789.1007916

In vitro antimicrobial estimation of some methyl 2-methyl-6-oxo-4-phenyl-1,4,5,6-tetrahydropyridine-3- carboxylate derivatives

Articles in Press

Document Type : Original Research Articles

Authors

Hojjat Sadeghi-aliabadi ¹

Marzieh Sadeghipour ²

Maryam Abbasi ³

Shabnam Mahernia ⁴

Fatemeh Shafiee ²

¹ 1. Department of Pharmaceutical Chemistry, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran

² Isfahan University of Medical Sciences

³ Department of Pharmaceutical Chemistry, School of Pharmacy and Pharmaceutical sciences, Hormozgan University of Medical Sciences, Bandar Abbas, Iran

⁴ The Institute of Pharmaceutical Sciences (TIPS), Tehran University of Medical Sciences, Tehran, Iran

10.22059/jsciences.2025.391789.1007916

Abstract

Abstract:
Compounds derived from pyridine are important chemicals with extensive use in various fields. The present study aims to investigate the toxicity, antimicrobial, and antifungal properties of newly synthesized pyridine derivatives. The broth microdilution method was used to determine the antimicrobial effects against Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella pneumoniae, Escherichia coli, Samonella typhi, and Candida albicans, obtained from the PTCC. MTT assay was used to determine the cytotoxicity of eight pyridine derivatives against HEPG2 and HT-29 and also HUVEC as a normal cell line. The results showed that compounds (2), (7), and (8) showed the most antimicrobial effects (MIC < 83.3 µg/mL), with no significant difference between the susceptibility of various microorganisms toward evaluated compounds. Results showed that most compounds were not cytotoxic, and only in the highest concentration (100 µg/mL) showed some toxicity against cancer cells in comparison to the negative control. As a conclusion, Compound (8) shows potential as an active ingredient for further investigation of its various biological activities. However, the structure-activity relationship is essential for determining vital substitutions and finding more suitable replacements for enhanced biological activity.

Keywords

Pyridine derivatives

Antimicrobial

MIC

Cytotoxicity

MTT assay

Subjects