PRODUCTION OF BETA-GALACTOSIDASE IN SUBMERGED MEDIA BY ASPERGILLUS ORYZAE, PTCC 5 163

Abstract
Different compositions of liquid media were used for their efficacy in the
production of beta-galactosidase by Aspergillus oryzae in submerged fermentation.
Enzyme production was improved by switching from lactose-based media to soybean
meal- and wheat bran-based media. Further improvement was made when the
soybean meal medium was supplemented with sodium nitrate and magnesium
sulfate. Results also indicate that fungal extracellular beta-galactosidase production
occurs concomitantly with growth on complex media but not on lactose- based media.
The effectiveness of Tween 80 on beta-galactosidase activity was found to depend on
the type of the medium used