PREPARATION AND APPLICATIONS OF FLUORESCENT POLYGLUTARALDEHYDE IMMUNOMICROSPHERES

Abstract
Aqueous glutaraldehyde has been polymerized under alkaline conditions
in the presence of a surfactant to yield microspheres of varied
diameters. Microbeads of a nominal 300 nm diameter, made fluorescent
via fluoresceinisothiocyanate, were used as a carrier of rabbit anti-human
red blood cell antibodies. Specific labeling of the human red blood cells as
well as diagnostic use of these fluorescent immunomicrospheresin detection
of brucella antibody or antigen has been clearly demonstrated.