STUDIES ON THE TISSUE SPECIFICITY OF COLONY STIMULATING FACTOR

Abstract

In this study granulocyte-macrophage colony stimulating factor (CSF) was
prepared from rabbit and guinea pig lung tissues. The biological activity of these
CSFs was measured on semisolid agar culture and compared with those of mouse
and rat. Weight of animals, incubation time and cross-reactivity of CSFs with
different bone marrow sources were also investigated. The results showed that both
rabbit and guinea pig lung tissues produced and released CSF in the conditioned
medium. The CSFs were active on ammonium chloride treated bone marrow cells
from balblc mouse and on their own ammonium chloride treated bone marrow cells
but could not stimulate the progenitor cells from other sources