Abstract

Cellular and tissue activities highly depend on environmental pH. Murine lung
tissue, when cultured properly in-vitro, is a potent producer of hematopoietic
growth factors. We have studied the effect of pH on the production of hematopoietic
growth factors and protein synthesis by the murine lung in-vitro. Various
concentrations of NaHC03 were used to adjust the pH of the culture medium under
constant CO, partial pressure. The results indicated that hematopoietic growth factor
production and protein synthesis by the lung were pH dependent. This dependency
was not a result of variations in cation (N concentration in the culture medium
because N reconstituted samples showed the same pattern of dependency on pH
as did the unreconstituted samples. Maximum rate of protein synthesis by the lung
was at pH 7. 6. However, the maximum rate of specific production of hematopoietic
growth factors was observed at pH 8