EFFECTS OF TEMPERATURE AND PERCENTAGE OF ORGANIC MODIFIER ON RETENTION  AND SELECTIVITY IN RP-HPLC USING  SOLVATION PARAMETER MODEL

Hadjmohammadi, M.R.

EFFECTS OF TEMPERATURE AND PERCENTAGE OF ORGANIC MODIFIER ON RETENTION AND SELECTIVITY IN RP-HPLC USING SOLVATION PARAMETER MODEL

Document Type : Original Research Articles

Author

M.R. Hadjmohammadi

Reversed-phase liquid chromatography; Solvation parameter model

Abstract

Effects of temperature and percentage of organic modifier were studied on retention and selectivity in RP-HPLC using solvation parameter model. The system constants were determined by multiple linear regression analysis from experimental values in the retention factor for a group of different solutes with known descriptors by computer using the program SPSS/PC. The experimental results showed that the variation of percentages of organic modifier (5-20% V/V CH₃CN) changes cavity formation (m = +3.55 to +2.53), dipole-dipole interactions (s = +0.62 to −0.64) and hydrogen-bond acidity (b = −2.41 to −3.27) terms. The variation of temperature (5-45°C) also changes cavity formation
(m = +3.21 to +2.56) and dipole-dipole interactions (s = +0.34 to −0.22) terms.

Keywords

Reversed-phase liquid chromatography

Solvation parameter model