Cellulolytic complex are enzymes capable of hydrolyzing cellulose. Due to rapid growth in population and industrialization, most countries are required to produce more fuel. Production of bioethanol from lignocellulosic biomass is very challenging due to environmental pollution by fossil fuels. Cellulases play a significant role in biotechnological processes. The cost of production of cellulase is very high. Approximately 50% of the cost of producing bioethanol is used to produce cellulose. Gibberellin regulates the growth of fungi and plants. Cellulase production was significantly improved by gibberellins hormone. The cellulase activity was 4.29 FPU/ml (when we used 50 μmol/ml of gibberellins in culture medium). This was 12- fold higher (0.34 FPU/ml in control solution) compare with untreated hormone and was comparable to that achieved with concentrated by n- butanol. In this experiment, the enzyme activity was increased 223- fold higher compare with control solution.