Document Type : Original Paper
Authors
- Atefeh Mir 1
- M. Amin tabatabaiefar 2
- Seyedeh Sedigheh Baakhlagh 3
- Hoora Jalali Tehrani 4
- Mahdi Zamani 3
1 ¹ Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, Tehran, Islamic Republic of Iran ² Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Islamic Republic of Iran 2.Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
2 ² Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Islamic Republic of Iran
3 ¹ Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, Tehran, Islamic Republic of Iran
4 ³ Department of Developmental Biology, Faculty of Advanced Sciences and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Islamic Republic of Iran
Abstract
Tissue regeneration is the process of renewal and growth of damaging tissue. It is sometimes done by induction of an external biological factor(s) such as the Basic fibroblast growth factor (bFGF) which carries messages through its cellular receptor to induce biological processes such as cell growth, cell migration, cell survival, and cell differentiation. Due to the great importance of bFGF on cellular regeneration, in this study, we have concentrated on the effect of this factor on the regeneration of damaged muscle. In addition, the tumorigenic effect of this factor during this regenerative process was studied. In this study, we employed inbred mice. The cultured male mouse muscle cells were transplanted into the damaged muscle tissues of HLA_matched female mice. After about one month, selected samples from the transplanted regions of the female mice were analyzed. DNA was extracted from the samples and Sex determination was done to track the potential cell growth and repair by the introduced male cells in the presence and absence of bFGF. The tumorigenic effect of bFGF on this process was also assessed. We found that bFGF had a remarkable effect on damaged muscle regeneration compared to cells without injection of this factor, and more concentration of bFGF was determined to be more effective in muscle regeneration. However, no association was observed with tumorigenesis regarding bFGF injection. According to our findings, bFGF was effective in the regeneration of the injury site and confirmed the results of previous studies. However, no association was found with tumorigenesis.
Keywords
Main Subjects
- Egner IM, Bruusgaard JC, Gundersen K. Satellite cell depletion prevents fiber hypertrophy in skeletal muscle. Development. 2016;143(16):2898-906.
- Gayraud-Morel B, Chrétien F, Tajbakhsh S. Skeletal muscle as a paradigm for regenerative biology and medicine. Regen Med. 2009 Mar;4(2):293-319.
- Zammit PS, Partridge TA, Yablonka-Reuveni Z. The skeletal muscle satellite cell: the stem cell that came in from the cold. J Histochem Cytochem. 2006;54(11):1177-91.
- Arnold L, Henry A, Poron F, Baba-Amer Y, Van Rooijen N, Plonquet A, et al. Inflammatory monocytes recruited after skeletal muscle injury switch into antiinflammatory macrophages to support myogenesis. J Experim Med. 2007;204(5):1057-69.
- Abou-Khalil R, Mounier R, Chazaud B. Regulation of myogenic stem cell behaviour by vessel cells: The" ménage à trois" of satellite cells, periendothelial cells and endothelial cells. Cell Cycle. 2010;9(5):892-6.
- Murphy MM, Lawson JA, Mathew SJ, Hutcheson DA, Kardon G. Satellite cells, connective tissue fibroblasts and their interactions are crucial for muscle regeneration. Development. 2011;138(17):3625-37.
- Gurtner GC, Werner S, Barrandon Y, Longaker MT. Wound repair and regeneration. Nature. 2008;453(7193):314-21.
- Hay ED. Cell biology of extracellular matrix: Springer Science & Business Media; 2013.
- Yun Y-R, Won JE, Jeon E, Lee S, Kang W, Jo H, et al. Fibroblast growth factors: biology, function, and application for tissue regeneration. J Tissue Eng. 2010;1(1):218142.
- Lichtman MK, Otero‐Vinas M, Falanga V. Transforming growth factor beta (TGF‐β) isoforms in wound healing and fibrosis. Wound Repair Regenera. 2016;24(2):215-22.
- Smith C, Klaasmeyer J, Woods T, Jones S. Effects of IGF-I, IGF-II, bFGF and PDGF on the initiation of mRNA translation in C2C12 myoblasts and differentiating myoblasts. Tissue Cell. 1999;31(4):403-12.
- Springer ML, Chen AS, Kraft PE, Bednarski M, Blau HM. VEGF gene delivery to muscle: potential role for vasculogenesis in adults. Mol Cell. 1998;2(5):549-58.
- Bertrand‐Duchesne MP, Grenier D, Gagnon G. Epidermal growth factor released from platelet‐rich plasma promotes endothelial cell proliferation in vitro. J Period Res. 2010;45(1):87-93.
- Eswarakumar V, Lax I, Schlessinger J. Cellular signaling by fibroblast growth factor receptors. Cytokine Growth Factor Rev. 2005;16(2):139-49.
- Hwang JH, Kim IG, Piao S, Jung AR, Lee JY, Park KD, et al. Combination therapy of human adipose-derived stem cells and basic fibroblast growth factor hydrogel in muscle regeneration. Biomaterials. 2013;34(25):6037-45.
- Doukas J, Blease K, Craig D, Ma C, Chandler LA, Sosnowski BA, et al. Delivery of FGF genes to wound repair cells enhances arteriogenesis and myogenesis in skeletal muscle. Mol Ther. 2002;5(5):517-27.
- Mitchell CA, McGeachie JK, Grounds MD. The exogenous administration of basic fibroblast growth factor to regenerating skeletal muscle in mice does not enhance the process of regeneration. Growth Factors. 1996;13(1-2):37-55.
- Schultz E, Jaryszak DL, Valliere CR. Response of satellite cells to focal skeletal muscle injury. Muscle Nerve. 1985;8(3):217-22.
- Ceafalan LC, Popescu BO, Hinescu ME. Cellular players in skeletal muscle regeneration. BioMed Res Int. 2014;2014.
|
|
- Brzoska E, Kowalewska M, Markowska‐Zagrajek A, Kowalski K, Archacka K, Zimowska M, et al. Sdf‐1 (CXCL12) improves skeletal muscle regeneration via the mobilisation of Cxcr4 and CD34 expressing cells. Biol Cell. 2012;104(12):722-37.
- Ratajczak MZ, Majka M, Kucia M, Drukala J, Pietrzkowski Z, Peiper S, et al. Expression of functional CXCR4 by muscle satellite cells and secretion of SDF‐1 by muscle‐derived fibroblasts is associated with the presence of both muscle progenitors in bone marrow and hematopoietic stem/progenitor cells in muscles. Stem Cells. 2003;21(3):363-71.
- Allen RE, Boxhorn LK. Regulation of skeletal muscle satellite cell proliferation and differentiation by transforming growth factor‐beta, insulin‐like growth factor I, and fibroblast growth factor. J Cell Physiol. 1989;138(2):311-5.
- Syverud BC, VanDusen KW, Larkin LM. Growth factors for skeletal muscle tissue engineering. Cells Tissues Organs. 2016;202(3-4):169-79.
- Karp JM, Teo GSL. Mesenchymal stem cell homing: the devil is in the details. Cell Stem Cell. 2009;4(3):206-16.
- Naderi‐Meshkin H, Bahrami AR, Bidkhori HR, Mirahmadi M, Ahmadiankia N. Strategies to improve homing of mesenchymal stem cells for greater efficacy in stem cell therapy. Cell Biol Int. 2015;39(1):23-34.
- Kasemkijwattana C, Menetrey J, Somogyi G, Moreland MS, Fu FH, Buranapanitkit B, et al. Development of approaches to improve the healing following muscle contusion. Cell Tansplant. 1998;7(6):585-98.
- Menetrey J, Kasemkijwattana C, Day C, Bosch P, Vogt M, Fu F, et al. Growth factors improve muscle healing in vivo. J Bone Joint Surg Br Vol. 2000;82(1):131-7.
- Bamman MM, Shipp JR, Jiang J, Gower BA, Hunter GR, Goodman A, et al. Mechanical load increases muscle IGF-I and androgen receptor mRNA concentrations in humans. Am J Physiol Endocrinol Metab. 2001;280(3):E383-E90.
- Allen RE, Dodson MV, Luiten LS. Regulation of skeletal muscle satellite cell proliferation by bovine pituitary fibroblast growth factor. Experim Cell Res. 1984;152(1):154-60.
- Gospodarowicz D, Moran JS. Mitogenic effect of fibroblast growth factor on early passage cultures of human and murine fibroblasts. J Cell Biol. 1975;66(2):451-7.
- Kasemkijwattana C, Menetrey J, Bosch P, Somogyi G, Moreland MS, Fu FH, et al. Use of growth factors to improve muscle healing after strain injury. Clin Orthopaed Relat Res. 2000;370:272-85.
- Grose R, Dickson C. Fibroblast growth factor signaling in tumorigenesis. Cytokine Growth Factor Rev. 2005;16(2):179-86.
- Marx RE. Dental and craniofacial applications of platelet-rich plasma: Quintessence Publishing (IL); 2005.
- Marx RE. Platelet-rich plasma: evidence to support its use. J Oral Maxillofac Surg. 2004;62(4):489-96.
)